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Journal of Neuro-Oncology

, Volume 108, Issue 1, pp 45–52 | Cite as

A histopathological diagnostic marker for human spinal astrocytoma: expression of glial fibrillary acidic protein-δ

  • Dong Hwa Heo
  • Se Hoon Kim
  • Kyung-Moo Yang
  • Yong Jun Cho
  • Keung Nyun Kim
  • Do Heum Yoon
  • Tae-Cheon KangEmail author
Laboratory Investigation

Abstract

Although histopathological diagnosis of spinal cord astrocytomas is important for postoperative treatment planning and prognosis, there is a lack of reliable immunohistochemical markers. The purpose of our study was to assess the expression pattern of GFAP-δ in spinal cord astrocytomas in human patients and to evaluate the utility of GFAP-δ as an immunohistochemical diagnostic marker. A total of 22 patients with spinal cord astrocytic tumors were included in this study. Patients were classified according to the WHO designation of human astrocytic tumors; three patients had grade 1 astrocytomas, 14 had grade 2, and five had Grade 3. Normal control spinal cord tissues were obtained at autopsy from the cervical spinal cords of ten patients with no history of cervical trauma or neurological disease. We evaluated BRAF, IDH1, GFAP, and GFAP-δ immunoreactivity in control tissues and astrocytomas. In normal control tissues, GFAP immunoreactivity was detected in astrocytes whereas GFAP-δ immunoreactivity was observed in very few astrocytes adjacent to the subpial layer of the spinal cord. GFAP-δ immunoreactivity was significantly correlated with spinal cord astrocytoma grade in astrocytomas compared to that in normal control tissues. The optical density of GFAP-δ increased significantly with astrocytoma grade (correlation coefficient, R 2 = 0.680). Also, BRAF and IDH1 immunoreactivity were detected in astrocytoma. We suggest that GFAP-δ may be an additional, reliable histopathological diagnostic marker for spinal cord astrocytomas.

Keywords

Astrocytic tumor Astrocyte Glial fibrillary acidic protein-δ Spinal cord 

Abbreviations

GFAP-δ

Human glial fibrillary acidic protein-delta

BRAF

Human v-raf murine sarcoma viral oncogene homolog B1 gene

IDH1

Human cytosolic NADP+ dependent isocitrate dehydrogenase 1

WHO

World Health Organization

PBS

Phosphate-buffered saline

aa

Amino acid

Notes

Acknowledgments

This study was supported by the BioGreen21 Program (200810FTH010103002 and 200810FTH010102001) of the Rural Development Administration.

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  • Dong Hwa Heo
    • 6
  • Se Hoon Kim
    • 1
  • Kyung-Moo Yang
    • 2
  • Yong Jun Cho
    • 6
  • Keung Nyun Kim
    • 3
  • Do Heum Yoon
    • 3
  • Tae-Cheon Kang
    • 4
    • 5
    Email author
  1. 1.Department of Pathology, College of MedicineYonsei UniversitySeoulKorea
  2. 2.Department of Forensic MedicineNational Forensic ServiceSeoulKorea
  3. 3.Department of Neurosurgery, College of MedicineSeverance Hospital, Yonsei UniversitySeoulKorea
  4. 4.Department of Anatomy & Neurobiology, College of MedicineHallym UniversityChuncheonKorea
  5. 5.Institute of Epilepsy Research, College of MedicineHallym UniversityChuncheonKorea
  6. 6.Department of Neurosurgery, College of MedicineChuncheon Sacred Heart Hospital, Hallym UniversityChuncheonKorea

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