Journal of Neuro-Oncology

, Volume 106, Issue 1, pp 53–58 | Cite as

The genotoxic effect of radiofrequency waves on mouse brain

  • Emin KaracaEmail author
  • Burak Durmaz
  • Huseyin Altug
  • Teoman Yildiz
  • Candan Guducu
  • Melis Irgi
  • Mehtap Gulcihan Cinar Koksal
  • Ferda Ozkinay
  • Cumhur Gunduz
  • Ozgur Cogulu
Laboratory Investigation - Human/Animal Tissue


Concerns about the health effects of radiofrequency (RF) waves have been raised because of the gradual increase in usage of cell phones, and there are scientific questions and debates about the safety of those instruments in daily life. The aim of this study is to evaluate the genotoxic effects of RF waves in an experimental brain cell culture model. Brain cell cultures of the mice were exposed to 10.715 GHz with specific absorbtion rate (SAR) 0.725 W/kG signals for 6 h in 3 days at 25°C to check for the changes in the micronucleus (MNi) assay and in the expression of 11 proapoptotic and antiapoptotic genes. It was found that MNi rate increased 11-fold and STAT3 expression decreased 7-fold in the cell cultures which were exposed to RF. Cell phones which spread RF may damage DNA and change gene expression in brain cells.


Radiofrequency Cell phone DNA Genotoxity 


  1. 1.
    Hoskote SS et al (2008) An epidemiological review of mobile telephones and cancer. J Assoc Phys India 56:980–984Google Scholar
  2. 2.
    Moulder JE et al (2005) Mobile phones, mobile phone base stations and cancer: a review. Int J Radiat Biol 81:189–203PubMedCrossRefGoogle Scholar
  3. 3.
    Hardell L et al (2006) Pooled analysis of two case-control studies on use of cellular and cordless telephones and the risk for malignant brain tumours diagnosed in 1997–2003. Int Arch Occup Environ Health 79:630–639PubMedCrossRefGoogle Scholar
  4. 4.
    Hardell L, Carlberg M (2009) Mobile phones, cordless phones and the risk for brain tumours. Int J Oncol 35:5–17PubMedCrossRefGoogle Scholar
  5. 5.
    Takebayashi T et al (2008) Mobile phone use, exposure to radiofrequency electromagnetic field, and brain tumour: a case-control study. Br J Cancer 98:652–659PubMedCrossRefGoogle Scholar
  6. 6.
    Akleman F, Sevgi L (1998) FDTD analysis of human head—mobile phone interaction in terms of specific absorption rate (SAR) calculations and antenna design. In: Proceedings of the conference on antennas and propagation for wireless communication, Waltham, USA, pp 85–88Google Scholar
  7. 7.
    Paker S, Sevgi L (1998) FDTD evaluation of the SAR distribution in a human head near a mobile cellular phone. Turk J Electron Commun 6:14–19Google Scholar
  8. 8.
    Fenech M, Morley AA (1985) Measurement of micronuclei in lymphocytes. Mutat Res 147(1–2):29–36PubMedGoogle Scholar
  9. 9.
    Kim JY et al (2008) In vitro assessment of clastogenicity of mobile-phone radiation (835 MHz) using the alkaline comet assay and chromosomal aberration test. Environ Toxicol 23:319–327PubMedCrossRefGoogle Scholar
  10. 10.
    Maes A et al (2006) Cytogenetic investigation of subjects professionally exposed to radiofrequency radiation. Mutagenesis 21:139–142PubMedCrossRefGoogle Scholar
  11. 11.
    Scarfì MR et al (2006) Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: an inter laboratory study. Radiat Res 165:655–663PubMedCrossRefGoogle Scholar
  12. 12.
    Schwarz C et al (2008) Radiofrequency electromagnetic fields (UMTS, 1, 950 MHz) induce genotoxic effects in vitro in human fibroblasts but not in lymphocytes. Int Arch Occup Environ Health 81:755–767PubMedCrossRefGoogle Scholar
  13. 13.
    Committee on Man, Radiation (COMAR) (2009) COMAR technical information statement: expert reviews on potential health effects of radiofrequency electromagnetic fields and comments on the bioinitiative report. Health Phys 97:348–356CrossRefGoogle Scholar
  14. 14.
    Campisi A et al (2010) Reactive oxygen species levels and DNA fragmentation on astrocytes in primary culture after acute exposure to low intensity microwave electromagnetic field. Neurosci Lett 473:52–55PubMedCrossRefGoogle Scholar
  15. 15.
    Thorlin T et al (2006) Exposure of cultured astroglial and microglial brain cells to 900 MHz microwave radiation. Radiat Res 166:409–421PubMedCrossRefGoogle Scholar
  16. 16.
    Diem E et al (2005) Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. Mutat Res 583:178–183PubMedGoogle Scholar
  17. 17.
    Lai H, Singh NP (2004) Magnetic-field-induced DNA strand breaks in brain cells of the rat. Environ Health Perspect 112:687–694PubMedCrossRefGoogle Scholar
  18. 18.
    Yang Y et al (2008) Case-only study of interactions between DNA repair genes (hMLH1, APEX1, MGMT, XRCC1 and XPD) and low-frequency electromagnetic fields in childhood acute leukemia. Leuk Lymph 49:2344–2350CrossRefGoogle Scholar
  19. 19.
    Khurana VG et al (2009) Cell phones and brain tumors: a review including the long-term epidemiologic data. Surg Neurol 72:205–214PubMedCrossRefGoogle Scholar
  20. 20.
    Nylund R, Leszczynski D (2006) Mobile phone radiation causes changes in gene and protein expression in human endothelial cell lines and the response seems to be genome and proteome dependent. Proteomics 6:4769–4780PubMedCrossRefGoogle Scholar
  21. 21.
    Zhao TY et al (2007) Exposure to cell phone radiation up-regulates apoptosis genes in primary cultures of neurons and astrocytes. Neurosci Lett 412:34–38PubMedCrossRefGoogle Scholar
  22. 22.
    Lagalla R et al (1996) An experimental in vitro evaluation of membrane antigen expression and of interleukin production by monocytes exposed to magnetic resonance. Radiol Med 91:292–296PubMedGoogle Scholar
  23. 23.
    Hao Y et al (2010) STAT3 signalling pathway is involved in the activation of microglia induced by 2.45 GHz electromagnetic fields. Int J Radiat Biol 86:27–36PubMedCrossRefGoogle Scholar
  24. 24.
    de la Iglesia et al (2008) Identification of a PTEN-regulated STAT3 brain tumor suppressor pathway. Genes Dev 22:449–462PubMedCrossRefGoogle Scholar
  25. 25.
    Tiwari R et al (2008) Combinative exposure effect of radio frequency signals from CDMA mobile phones and aphidicolin on DNA integrity. Electromagn Biol Med 27:418–425PubMedCrossRefGoogle Scholar
  26. 26.
    Rothman KJ (2000) Epidemiological evidence on health risks of cellular telephones. Lancet 356:1837–1840PubMedCrossRefGoogle Scholar
  27. 27.
    Snyder WS et al (1975) Report of the task group on reference man. Pergamon, OxfordGoogle Scholar
  28. 28.
    Erwin DN, Hurt WD (1981) Assessment of possible hazards associated with applications of millimeter-wave systems. Aeromedical review USAF-SAM 2-81. USAF School of Aerospace Medicine, Aerospace Medical Division, Brooks AFB, TXGoogle Scholar
  29. 29.
    Gandhi OP, Riazi A (1986) Absorption of millimeter waves by human beings and its biological implications. IEEE Trans Microw Theory Tech MTT 34:228–235CrossRefGoogle Scholar
  30. 30.
    Durney C (1986) Radiofrequency radiation dosimetry handbook, USAFSAM-TR 85-73. USAF School of Aerospace Medicine, Aerospace Medical Division, Brooks AFB, TXGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Emin Karaca
    • 1
    Email author
  • Burak Durmaz
    • 1
  • Huseyin Altug
    • 2
  • Teoman Yildiz
    • 3
  • Candan Guducu
    • 4
  • Melis Irgi
    • 4
  • Mehtap Gulcihan Cinar Koksal
    • 5
  • Ferda Ozkinay
    • 1
  • Cumhur Gunduz
    • 6
  • Ozgur Cogulu
    • 1
  1. 1.Department of Medical GeneticsEge University Faculty of MedicineIzmirTurkey
  2. 2.Department of Histology and EmbryologyEge University Faculty of MedicineIzmirTurkey
  3. 3.Department of PhysicsEge University Faculty of ScienceIzmirTurkey
  4. 4.High School of ScienceIzmirTurkey
  5. 5.Department of PharmacologyEge University Faculty of MedicineIzmirTurkey
  6. 6.Department of Medical BiologyEge University Faculty of MedicineIzmirTurkey

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