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Journal of Neuro-Oncology

, 94:333 | Cite as

‘Putting our heads together’: insights into genomic conservation between human and canine intracranial tumors

  • Rachael Thomas
  • Shannon E. Duke
  • Huixia J. Wang
  • Tessa E. Breen
  • Robert J. Higgins
  • Keith E. Linder
  • Peter Ellis
  • Cordelia F. Langford
  • Peter J. Dickinson
  • Natasha J. Olby
  • Matthew Breen
Laboratory Investigation - Human/animal tissue

Abstract

Numerous attributes render the domestic dog a highly pertinent model for cancer-associated gene discovery. We performed microarray-based comparative genomic hybridization analysis of 60 spontaneous canine intracranial tumors to examine the degree to which dog and human patients exhibit aberrations of ancestrally related chromosome regions, consistent with a shared pathogenesis. Canine gliomas and meningiomas both demonstrated chromosome copy number aberrations (CNAs) that share evolutionarily conserved synteny with those previously reported in their human counterpart. Interestingly, however, genomic imbalances orthologous to some of the hallmark aberrations of human intracranial tumors, including chromosome 22/NF2 deletions in meningiomas and chromosome 1p/19q deletions in oligodendrogliomas, were not major events in the dog. Furthermore, and perhaps most significantly, we identified highly recurrent CNAs in canine intracranial tumors for which the human orthologue has been reported previously at low frequency but which have not, thus far, been associated intimately with the pathogenesis of the tumor. The presence of orthologous CNAs in canine and human intracranial cancers is strongly suggestive of their biological significance in tumor development and/or progression. Moreover, the limited genetic heterogenity within purebred dog populations, coupled with the contrasting organization of the dog and human karyotypes, offers tremendous opportunities for refining evolutionarily conserved regions of tumor-associated genomic imbalance that may harbor novel candidate genes involved in their pathogenesis. A comparative approach to the study of canine and human intracranial tumors may therefore provide new insights into their genetic etiology, towards development of more sophisticated molecular subclassification and tailored therapies in both species.

Keywords

Comparative genomic hybridization Canine Brain tumor Chromosome Microarray 

Notes

Acknowledgements

We would like to acknowledge Pragna Mehta and the Veterinary Neurology and Pathology residents and clinicians from North Carolina State University and the University of California at Davis for assistance with tissue procurement. We thank Eric Seiser for assistance with data analysis. This work was supported by grants from the National Institutes of Health (NS051190) and the American Kennel Club Canine Health Foundation (CHF-403) awarded to MB and NJO. CFL and PE are supported by funds from the Wellcome Trust. HJW is supported by National Science Foundation Award (DMS-0706963). PD and RJH are supported by the Paul C and Borghild T Petersen Foundation.

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Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Rachael Thomas
    • 1
    • 2
  • Shannon E. Duke
    • 1
  • Huixia J. Wang
    • 3
  • Tessa E. Breen
    • 4
  • Robert J. Higgins
    • 5
  • Keith E. Linder
    • 2
    • 6
  • Peter Ellis
    • 7
  • Cordelia F. Langford
    • 7
  • Peter J. Dickinson
    • 8
  • Natasha J. Olby
    • 2
    • 4
  • Matthew Breen
    • 1
    • 2
    • 9
  1. 1.Department of Molecular Biomedical Sciences, College of Veterinary MedicineNorth Carolina State UniversityRaleighUSA
  2. 2.Center for Comparative Medicine and Translational ResearchNorth Carolina State UniversityRaleighUSA
  3. 3.Department of Statistics, College of Agriculture and Life SciencesNorth Carolina State UniversityRaleighUSA
  4. 4.Department of Clinical Sciences, College of Veterinary MedicineNorth Carolina State UniversityRaleighUSA
  5. 5.Department of Pathology, Microbiology and Immunology, School of Veterinary MedicineUniversity of California-DavisDavisUSA
  6. 6.Department of Population Health and Pathobiology, College of Veterinary MedicineNorth Carolina State UniversityRaleighUSA
  7. 7.Microarray FacilityThe Wellcome Trust Sanger InstituteCambridgeUK
  8. 8.Department of Surgical and Radiological Sciences, School of Veterinary MedicineUniversity of California-DavisDavisUSA
  9. 9.Cancer Genetics ProgramUNC Lineberger Comprehensive Cancer CenterChapel HillUSA

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