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Journal of Neuro-Oncology

, Volume 93, Issue 3, pp 325–332 | Cite as

miR-21 and 221 upregulation and miR-181b downregulation in human grade II–IV astrocytic tumors

  • Alfredo Conti
  • M’Hammed Aguennouz
  • Domenico La Torre
  • Chiara Tomasello
  • Salvatore Cardali
  • Filippo F. Angileri
  • Francesca Maio
  • Annamaria Cama
  • Antonino Germanò
  • Giuseppe Vita
  • Francesco Tomasello
Laboratory Investigation - Human/Animal Tissue

Abstract

MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. Recent evidence indicates that miRNAs can function both as tumor suppressors and as oncogenes. It has been demonstrated that in glioblastoma multiforme miR-21 and 221 are upregulated whereas miR-128 and 181 are downregulated. Expression of miR-21, 221, 128a, 128b, 128c, 181a, 181b, 181c was studied using real-time quantitative reverse transcriptase polymerase chain reaction and northern blotting for human astrocytic tumors with different grade of malignancy. miR-21 and 221 were overexpressed in glioma samples, whereas miRNA 181b was downregulated compared with normal brain tissue. miRNA-21 was hyperexpressed in all tumor samples whereas higher levels of miRNA-221 were found in high-grade gliomas. This study is the first analysis of miRNAs in astrocytic tumor at different stages of malignancy. The different expression pattern observed in tumors at different stages of malignancy is probably dependent on the cell-specific repertoire of target genes of tumors sharing different molecular pathways activity and suggests miRNAs may have also a place in diagnosis and staging of brain tumors.

Keywords

MicroRNA miR-21 miR-221 miR-181 Glioma Brain tumor 

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Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Alfredo Conti
    • 1
  • M’Hammed Aguennouz
    • 2
  • Domenico La Torre
    • 1
  • Chiara Tomasello
    • 3
  • Salvatore Cardali
    • 1
  • Filippo F. Angileri
    • 1
  • Francesca Maio
    • 4
  • Annamaria Cama
    • 4
  • Antonino Germanò
    • 1
  • Giuseppe Vita
    • 2
  • Francesco Tomasello
    • 1
  1. 1.Neurosurgery, Department of NeuroscienceA.O.U. Policlinico Universitario, University of MessinaMessinaItaly
  2. 2.Department of NeurologyUniversity of MessinaMessinaItaly
  3. 3.Department of OncologyUniversity of MessinaMessinaItaly
  4. 4.Neuro-oncology PhD ProgramUniversity of MessinaMessinaItaly

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