Journal of Neuro-Oncology

, Volume 85, Issue 1, pp 49–63 | Cite as

Quantifying the A1AR distribution in peritumoural zones around experimental F98 and C6 rat brain tumours

  • Markus Dehnhardt
  • Christoph Palm
  • Andrea Vieten
  • Andreas Bauer
  • Uwe Pietrzyk
Lab. Investigation-human/animal tissue


Quantification of growth in experimental F98 and C6 rat brain tumours was performed on 51 rat brains, 17 of which have been further assessed by 3D tumour reconstruction. Brains were cryosliced and radio-labelled with a ligand of the peripheral type benzodiazepine-receptor (pBR), 3H-Pk11195 [(1-(2-chlorophenyl)-N-methyl-N-(1-methyl-propylene)-3-isoquinoline-carboxamide)] by receptor autoradiography. Manually segmented and automatically registered tumours have been 3D-reconstructed for volumetric comparison on the basis of 3H-Pk11195-based tumour recognition. Furthermore automatically computed areas of −300 μm inner (marginal) zone as well as 300 μm and 600 μm outer tumour space were quantified. These three different regions were transferred onto other adjacent slices that had been labelled by receptor autoradiography with the A1 Adenosine receptor (A1AR)-ligand 3H-CPFPX (3H-8-cyclopentyl-3-(3-fluorpropyl)-1-propylxanthine) for quantitative assessment of A1AR in the three different tumour zones. Hence, a method is described for quantifying various receptor protein systems in the tumour as well as in the marginal invasive zones around experimentally implanted rat brain tumours and their representation in the tumour microenvironment as well as in 3D space. Furthermore, a tool for automatically reading out radio-labelled rat brain slices from auto radiographic films was developed, reconstructed into a consistent 3D-tumour model and the zones around the tumour were visualized. A1AR expression was found to depend upon the tumour volume in C6 animals, but is independent on the time of tumour development. In F98 animals, a significant increase in A1AR receptor protein was found in the Peritumoural zone as a function of time of tumour development and tumour volume.


3D reconstruction A1 adenosine receptor GBM Kmeans algorithm Brain tumour Receptor autoradiography 



A1 adenosine receptor


Cloned rat glial tumour cell line




Astrocytic tumour cell line


Glioblastoma multiforme




Peripheral-type benzodiazepine receptor




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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Markus Dehnhardt
    • 1
    • 2
  • Christoph Palm
    • 1
    • 3
  • Andrea Vieten
    • 1
  • Andreas Bauer
    • 1
  • Uwe Pietrzyk
    • 1
  1. 1.Institute of Neuroscience and Biophysics 3-Medicine, Research Centre JuelichJuelichGermany
  2. 2.Medac GmbHWedelGermany
  3. 3.Centre for Medical Image Computing (CMIC)University College LondonLondonUnited Kingdom

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