Journal of Neuro-Oncology

, Volume 76, Issue 2, pp 99–104 | Cite as

Targeted Tumor Cell Death Induced by Autologous Tumor-Specific T Lymphocyte Recognition of Wild-Type p53-Derived Peptides

  • Hideo Tsurushima
  • Yoshihiko Yoshii
  • Kam W Leong
  • Tadao Ohno
Laboratory Investigation

Summary

Autologous tumor-specific T lymphocyte (ATTL) lines were derived from the peripheral blood mononuclear cells (PBMC) of a healthy volunteer with human leukocyte antigen (HLA) -A*0201. These lines were achieved using interleukins -1β, -2, -4, and -6 and the p53-based peptide from the 264–272 sequence of the wild-type p53 protein with a strong affinity against HLA-A*0201.

;The frequencies of CD3+, CD4+, and CD8+ lymphocytes were 94–96%, 30–34%, and 69–74%, respectively. ATTLs killed most of the T2 cells pulsed with p53-derived peptide, but not against the T2 cells non-pulsed or pulsed with an irrelevant peptide. ATTLs also killed TKB-14 cells, which have been derived from human glioblastoma multiforme, and exhibited HLA-A*0201 molecule and immunohistochemical accumulation of p53 protein. These cytotoxic activities were inhibited by anti-CD3, anti-CD8, and anti-class I antibodies.

These findings suggested that these ATTL lines might include CTL populations, which could recognize p53-derived peptide on HLA-A*0201 and the p53-based peptide may play as an antigen on HLA-A*0201. When tumor antigens would be more analyzed in the future, ATTL could be induced without the primary-cultured cells from tumor tissue and could be applied for cancer therapy.

Keywords

antigen presentation cytotoxic T lymphocyte glioblastoma multiforme p53-immunopeptide 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Hideo Tsurushima
    • 1
    • 2
    • 3
  • Yoshihiko Yoshii
    • 1
  • Kam W Leong
    • 3
  • Tadao Ohno
    • 2
  1. 1.Faculty of Medicine, Department of Neuro SurgeryUniversity of the RyukyusOkinawaJapan
  2. 2.RIKEN Cell Bank, RIKENThe Institute of Physical and Chemical ResearchTsukuba Science CityJapan
  3. 3.Department of Biomedical Engineering, School of MedicineJohns Hopkins UniversityUSA
  4. 4.Nanotechnology Research Institute (NRI)National Institute of Advanced Industrial Science and Technology (AIST)IbarakiJapan

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