Genetic diversity and differentiation of yellowwood [Cladrastis kentukea (Dum.Cours.) Rudd] growing in the wild and in planted populations outside the natural range
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Yellowwood (Cladrastis kentukea) grows in small, widely scattered populations in the wild, but is also a popular ornamental tree that thrives when planted in urban areas outside its natural range. Since the small native populations of yellowwood in several states are considered at risk of extirpation, the cultivated population could serve as an ex situ repository of yellowwood genetic diversity that could be used to restore lost local populations of the species. The potential value of cultivated yellowwood for conservation depends on the genetic diversity among cultivated trees compared to natural populations. Using nuclear microsatellite markers, we genotyped 180 yellowwoods from natural populations in Indiana, Missouri, Arkansas, and Kentucky, along with 61 trees from urban parks and landscapes in Indiana, Ohio, and Missouri. We found that, even when statistics were adjusted based on population size, the urban “population” had higher genetic diversity than any of the wild populations sampled, indicating that commercially-grown yellowwood is most likely a mixture of genotypes from isolated wild populations. We observed strong genetic differentiation among wild populations, and evidence for inbreeding in at least one of the wild populations.
KeywordsEx situ conservation Urban forest Landscape genetics Fabaceae
The Nature Conservancy provided funding for the work described in this paper. The authors would like to thank Megan Simmons for her help with lab work and genotyping. The authors would also like to thank all those who helped us find sampling sites in Indiana, Kentucky, and Arkansas, and all those who mailed us yellowwood samples from Missouri and Ohio.
- Andrianasolo DN, Davis AP, Razafinarivo NJ, Hamon S, Rakotomalala J-J, Sabatier S-A, Hamon P (2013) High genetic diversity of in situ and ex situ populations of Madagascan coffee species: further implications for the management of coffee genetic resources. Tree Genet Genomes 9:1295–1312CrossRefGoogle Scholar
- Burczyk J, DiFazio SP, Adams WT (2004) Gene flow in forest trees: how far do genes really travel? For Genet 11(3–4):179–192Google Scholar
- Christe C, Kozlowski G, Frey D, Fazan L, Betrisey S, Pirintsos S, Gratzfeld J, Naciri Y (2014) Do living ex situ collections capture the genetic variation of wild populations? A molecular analysis of two relict tree species, Zelkova abelica and Zelkova carpinifolia. Biodivers Conserv 23:2945–2959CrossRefGoogle Scholar
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- Gilkison VA (2013) Comparisons of genetic diversity among disjunct populations of Magnolia tripetala. Western Kentucky University Honors College Capstone Experience/Thesis Projects. Paper 423. http://digitalcommons.wku.edu/stu_hon_theses/423
- Hill SR (2007) Conservation assessment for yellowwood (Cladrastis kentukea (Dum. Cours.) Rudd) INHS Technical Report 2007 (28), 7 May 2007, p 33Google Scholar
- Kubisiak TL, Roberds JH (2005) Genetic structure of natural populations based on neutral DNA markers. In: Steiner KC, Carlson JE (eds) Proceedings of conference on restoration of American chestnut to forest landsGoogle Scholar
- Leopold DJ, McComb WC, Muller RN (1998) Trees of the central hardwood forests of North America. Timber Press, Portland. ISBN 0-88192-406-7Google Scholar
- Peattie DC (1950) A natural history of trees of eastern and central North America. Houghton Mifflin, Boston, p 1950Google Scholar