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New Forests

, Volume 42, Issue 2, pp 179–193 | Cite as

Patterns of genetic diversity that result from bottlenecks in Scots Pine and the implications for local genetic conservation and management practices in Bulgaria

  • Krassimir D. NaydenovEmail author
  • Michel K. Naydenov
  • Francine Tremblay
  • Alexander Alexandrov
  • Louis Daniel Aubin-Fournier
Article

Abstract

As one of the principal coniferous species of commercial importance in Bulgaria, Scots Pine has been subjected to strict management, which has posed a challenge for the conservation and management of its gene pool. In this study, we measured genetic diversity and population structure using 6 microsatellite loci in 12 populations from Bulgarian mountain ranges. Our analysis showed good intrapopulation divergence (18.22%; AMOVA) and genetic evidence of bottlenecks. “NJ-IAM” and “non-admixing” clusters divided the populations into 3 large groups. Our microsatellite analysis was consistent with the documented “mountain effect” influence on genetic diversity. Two methods—the Coalescent Bayesian model and M-stat analysis—implicated recent bottlenecks for all populations, suggesting that the genetic structure of Bulgarian Scots Pine was shaped over a long period (one thousand generations; i.e. 20,000 years) by the interaction of genetic and autoecological processes. Our results suggest that conservation and management practices should strive to maintain this genetic differentiation, specifically by emphasizing reforestation efforts with stocks from local provenances to avoid nonlocal introductions.

Keywords

Nuclear microsatellite markers Pinussylvestris L. Bottleneck Effective population size Biogeography 

Notes

Acknowledgments

We thank Irena M. Naydenova and M. H. Longpré for their technical assistance. We are grateful to the staff of the Bulgarian forest service for their assistance with the plant material; the Ministry of Education and Science of Bulgaria for their financial support (CC-318/1993–1996); and the Fond KAZAROVI-Geneva (Switzerland) for a student grant to K. D. Naydenov during an internship at the University of Geneva (Department of Botany). We also wish to thank W. Parsons (University of Sherbrook) and BLUE PENCIL-SCIENCE (USA) for his careful revision of this manuscript. This study was funded by a post-doctoral grant to K. D. Naydenov from the “Chaire en aménagement forestier durable”, while other financial support was provided by a Natural Sciences and Engineering Research Council of Canada (NSERC) operating grant to F. Tremblay.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Krassimir D. Naydenov
    • 1
    Email author
  • Michel K. Naydenov
    • 1
    • 2
    • 3
  • Francine Tremblay
    • 1
  • Alexander Alexandrov
    • 4
  • Louis Daniel Aubin-Fournier
    • 1
  1. 1.Chaire CRSNG-UQAT-UQAM en aménagement forestier durable, Centre d’étude de la forêtUniversité du Québec en Abitibi-TémiscamingueRouyn-NorandaCanada
  2. 2.Lycee de langue francaise “Alphonse de Lamartine”SofiaBulgarie
  3. 3.Frenska Ezikova Gimnazia Bulgaria. lycee N9SofiaBulgarie
  4. 4.Forest Research Institute, Bulgarian Academy of ScienceSofiaBulgaria, Europe

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