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Mating patterns in an indoor miniature Cryptomeria japonica seed orchard as revealed by microsatellite markers

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Abstract

To evaluate the practical application of an indoor seed orchard, we compared the seed production, final germination rates and mating patterns of potted Cryptomeria japonica D. Don clones in two miniature seed orchards: one in a glasshouse and the other outdoors. There was no statistically significant difference in total seed production between the orchards (P = 0.275, ANOVA). However, the final germination rate of seeds produced in the indoor orchard was significantly lower than that of seeds produced in the outdoor orchard (P < 0.01, Wilcoxon test). The average self-fertilization rate was higher in the indoor orchard (27.2%) than in the outdoor orchard (5.6%), and this parameter was strongly associated with the reduced final germination rates of the seeds harvested in the glasshouse. Pollen contamination was considerably lower in the indoor seed orchard (48.9% in the outdoor seed orchard and 4.4% in the indoor seed orchard). χ2 tests indicated that the paternal contributions of each constituent clone differed significantly in both orchards (P < 0.001 in both cases). The strong variations in paternal contributions among indoor orchard clones was significantly correlated with their respective pollen production capacities (Spearman ρ = 0.693, P < 0.001). To reduce the high self-fertilization rates, we recommend the use of orchard clones with similar pollen production capacity in indoor seed orchards.

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Acknowledgments

We thank M. Saito for his helpful advice. This work was partly supported by Research Fellowships from the Japan Society for the Promotion of Science for Young Scientists and Grant-in-Aid (Development of Technologies for Control of Pollen Production by Genetic Engineering) from the Forest Agency of Japan and Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry.

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Authors and Affiliations

  1. Department of Forest Genetics, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan

    Yoshinari Moriguchi & Yoshihiko Tsumura

  2. Graduate School of Science and Technology, Niigata University, Igarashi 2-nocho, Niigata, 950-2101, Japan

    Yoko Yamazaki & Hideaki Taira

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  1. Yoshinari Moriguchi
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  2. Yoko Yamazaki
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  3. Hideaki Taira
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  4. Yoshihiko Tsumura
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Correspondence to Yoshinari Moriguchi.

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Moriguchi, Y., Yamazaki, Y., Taira, H. et al. Mating patterns in an indoor miniature Cryptomeria japonica seed orchard as revealed by microsatellite markers. New Forests 39, 261–273 (2010). https://doi.org/10.1007/s11056-009-9169-0

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  • DOI: https://doi.org/10.1007/s11056-009-9169-0

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