Neuroscience and Behavioral Physiology

, Volume 49, Issue 9, pp 1195–1198 | Cite as

The Sleep–Waking Cycle and Experimental Models of Mutations in the Panx1 Gene

  • V. M. KovalzonEmail author
  • A. A. Latyshkova
  • A. D. Komarova
  • Yu. V. Panchin

Objectives. To confirm the authors’ previous suggestion that mutations in the Panx1 gene (pannexin-1) may play an important role in the development of impairments to the sleep-waking cycle. Materials and methods. Studies were carried out using mice with knockout of the Panx1 gene. Experiments used continuous 24-h EEG recording of the EEG and motor activity. Results and conclusions. A statistically significant increase in the proportion of waking was found, due to a decrease in the slow-wave sleep phase (as compared with controls), particularly marked in the dark period of the day, along with an increase in motor activity. The results were compared with data obtained during investigation of a recently published case of a patient with a homozygous mutation in this gene. The possible physiological role of pannexin-1 protein in health and pathology is discussed.


sleep and waking Panx1 gene genetic diseases experimental models 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Q. Shao, K. Lindstrom, R. Shi, et al., “A germline variant in the PANX1 gene has reduced channel function and is associated with multisystem dysfunction, “ J. Biol. Chem., 291, No. 24, 12432–12443 (2016), Scholar
  2. 2.
    Y. Panchin, I. Kelmanson, M. Matz, et al., “A ubiquitous family of putative gap junction molecules, “ Curr. Biol., 10, No. 13, 473–474 (2000), Scholar
  3. 3.
    J. L. Esseltine and D. W. Laird, “Next-generation connexin and pannexin cell biology, “ Trends Cell Biol., 26, No. 12, 944–955 (2016), Scholar
  4. 4.
    V. I. Shestopalov and Y. V. Panchin, “Pannexins and gap junction protein diversity, “ Cell. Mol. Life Sci., 65, No. 3, 376–394 (2008), Scholar
  5. 5.
    S. C. Holst and H. P. Landolt, “Sleep homeostasis, metabolism, and adenosine, “ Curr. Sleep Med. Rep., 1, No. 1, 27–37 (2015), https:// Scholar
  6. 6.
    V. I. Shestopalov, Y. Panchin, O. S. Tarasova, et al., “Pannexins are potential new players in the regulation of cerebral homeostasis during sleep–wake cycle, “ Front. Cell. Neurosci., 11, 210 (2017), Scholar
  7. 7.
    G. Dvoriantchikova, D. Ivanov, D. Barakat, et al., “Genetic ablation of pannexin1 protects retinal neurons from ischemic injury, “ PLoS One, 7, e31991 (2012), Scholar
  8. 8.
    A. I. Manolov, V. M. Kovalzon, Yu. V. Ukraintseva, et al., “Dependence of the accuracy of automatic identification of sleep and waking states in mice on the spectral characteristics of the electroencephalogram, “ Zh. Vyssh. Nerv. Deyat., 65, No. 5, 635–640 (2015), Scholar
  9. 9.
    V. M. Kovalzon, L. S. Moiseenko, A. V. Ambaryan, et al., “Sleep–wakefulness cycle and behavior in pannexin1 knockout mice, “Behav. Brain Res., 318, 24–27 (2017), Scholar
  10. 10.
    R. Huber, T. Deboer, and I. Tobler, “Effects of sleep deprivation on sleep and sleep EEG in three mouse strains: empirical data and simulations, “ Brain Res., 857, 8–19 (2000), Scholar
  11. 11.
    T. Kim, V. Ramesh, M. Dworak, et al., “Disrupted sleep–wake regulation in type 1 equilibrative nucleoside transporter knockout mice, “ Neuroscience, 303, 211–219 (2015), neuroscience.2015.06.037.Google Scholar
  12. 12.
    С. Blanco-Centurion, M. Xu, E. Murillo-Rodriguez, et al., “Adenosine and sleep homeostasis in the basal forebrain, “ J. Neurosci., 26, 8092–8100 (2006), Scholar
  13. 13.
    NCBI, dbSNP, Short Genetic Variations,, acc. Feb. 21, 2017.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • V. M. Kovalzon
    • 1
    • 2
    Email author
  • A. A. Latyshkova
    • 2
    • 3
  • A. D. Komarova
    • 2
    • 3
  • Yu. V. Panchin
    • 2
    • 3
  1. 1.Severtsov Institute of Problems in Ecology and EvolutionRussian Academy of SciencesMoscowRussia
  2. 2.Kharkevich Institute of Problems in Information TransmissionRussian Academy of SciencesMoscowRussia
  3. 3.Lomonosov Moscow State UniversityMoscowRussia

Personalised recommendations