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Experimental Studies of the Expression of Neurotransmitter Transporter Genes in Astrocytes in Different Part of the Brain

  • N. N. Shusharina
  • M. V. Patrushev
  • E. V. SilinaEmail author
  • V. A. Stupin
  • P. F. Litvitsky
  • A. S. Orlova
Article
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Objectives. To study the expression of various neurotransmitter transporters (glutamate, aspartate, lactate, choline) in cultures of astrocytes from different part of the brain (cortex, hippocampus, and brainstem) in rats aged three and 11 days. Materials and methods. An experimental study was performed using 24 Rattus norvegicus rats aged three (n = 12) and 11 days (n = 12). High-throughput sequencing results were analyzed. Results. Expression of the glutamate and aspartate transporters in the brainstem in three-day-old rats was greater than in other areas, though the reverse pattern was seen in rats aged 11 days. Expression of the lactate transporter with age was identical to that in the cortex. Conclusions. The data obtained here demonstrated the nature of neuroastrocyte relationships and the role of astrocytes in the process of signal transmission. The results of the study, carried out using original methods of investigating neurotransmitter transporters, allow them to be recommended for monitoring the processes of neurogenesis and neurohomeostasis, including in cerebrovascular and neurodegenerative diseases.

Keywords

astrocytes neuroglia transporters neurotransmitters glutamate aspartate choline lactate gene expression genetic methods of recording neurogenesis 

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • N. N. Shusharina
    • 1
  • M. V. Patrushev
    • 1
  • E. V. Silina
    • 2
    Email author
  • V. A. Stupin
    • 3
  • P. F. Litvitsky
    • 3
  • A. S. Orlova
    • 3
  1. 1.Kant Baltic Federal UniversityKaliningradRussia
  2. 2.Sechenov First Moscow State Medical University (Sechenov University)MoscowRussia
  3. 3.Pirogov Russian National Research Medical University, Russian Ministry of HealthMoscowRussia

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