Methodological Features of the Morphometric Characterization of the Synaptoarchitectonics of the Human Neocortex by Immunofluorescent Detection of Neuromodulin
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Objective. To study the possibility of using GAP-43 for morphometric characterization of synaptoarchitectonics. Materials and methods. Studies used immunofluorescent detection of neuromodulin (GAP-43) by confocal microscopy and automated computer image analysis of layer V (field 4) of the human brain (n = 4) using ImageJ 1.46. Results. Immunofluorescent detection of GAP-43 identified the distribution of axodendritic and axosomatic synapses, the total area of axon terminals, and the number densities of intermediate and large terminals. Conclusions. A necessary condition for obtaining accurate data is having digital images with sufficient resolution (600–900 pixels per inch). These results need to be considered in studies of the synaptoarchitectonics of the neocortex using immunohistochemical methods for investigating synapse structure.
Keywordsneocortex synapses neuromodulin immunofluorescence automated computer analysis
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