Structural and Histochemical Changes in Neurons in the Frontal Cortex of the Brain in Rats with Cholestasis
- 7 Downloads
Objectives. To study structural and histochemical changes in neurons in the frontal cortex of the brain in rats after ligation of the common bile duct. Materials and methods. The cortex of the frontal lobe was studied in 72 mongrel adult white male rats weighing 200 ± 25 g. Ligation of the common bile duct and development of cholestasis (experimental group) and sham surgery (control group) was followed by histological and histochemical studies of microstructural changes in pyramidal neurons in all layers of the frontal cortex on days 2, 5, 10, 20, 45, and 90. Results. Ligation of the common bile duct in rats producing cholestasis led to profound histological and histochemical changes in neurons in the frontal cortex of the brain: changes in size and shape, and appearance of hyperchromic shrunken neurons and ghost cells. Impairments to energy and plastic metabolism in neurons led to the death of the experimental animals. The consequences of cholestasis in the brains of rats were apparent on days 2–5 and reached a maximum on days 10–20. In the later post-operative period (45–90 days), groups of survivors showed gradual reductions in these changes, though the consequences of cholestasis consisted of foci of neuron loss in all layers of the frontal cortex. Conclusions. It is suggested that survival of rats after ligation of the common bile duct is due to formation of bypass bile ducts, with elimination of cholestasis and the high adaptive potential of neurons.
Keywordscerebral cortex frontal cortex pyramidal neurons microstructural changes cholestasis
Unable to display preview. Download preview PDF.
- 1.S. V. Emel’yanchik and S. M. Zimatkin, The Brain in Cholestasis, Grodno State University, Grodno (2011).Google Scholar
- 2.S. V. Emel’yanchik and S. M. Zimatkin, “Structural and histochemical changes in cerebellar Purkinje cells in cholestasis,” Morfologiya, 143, No. 2, 19–23 (2013).Google Scholar
- 3.S. M. Zimatkin, O. V. Baraban, and S. V. Emel’yanchik, “Metabolic changes in rat brain histaminergic neurons during subhepatic cholestasis,” Morfologiya, 132, No. 4, 27–30 (2007).Google Scholar
- 4.Yu. S. Martynov, E. V. Malkova, and V. V. Proskurin, “Cholestatic toxic-vascular encephalopathy and encephalomyelopathy,” Zh. Nevrol. Psikhiat., 87, No. 11, 1640–1646 (1987).Google Scholar
- 5.E. Pearse, Histochemistry, Theoretical and Applied [Russian translation], Foreign Literature Press, Moscow (1962).Google Scholar
- 6.S. V. Chepur, Morphofunctional Characteristics of the Structure of the Nervous System in Health and Patterns of Changes in Hepatic Encephalopathy: Author’s Abstract of Doctoral Thesis in Medical Sciences, St. Petersburg State Pediatric Medical Academy, St. Petersburg (2003).Google Scholar
- 7.S. Sherlok and J. Dooley, Diseases of the Liver and Biliary System, GEOTAR Meditsina, MV (1999).Google Scholar
- 10.О. Н. Juarez, “Hepatic anatomopathologic injuries by extrahepatic biliary tract obstruction in mice,” Rev. Gastroenterol. Mex., 73, No. 1, 17–20 (2008).Google Scholar
- 11.L. A. Kikalishvili, “Morphological changes in brain and heart after the temporary liver exclusion from the bloodstream during the cholestasis,” Georgian Med. News, No. 167, 77–81 (2009).Google Scholar
- 12.F. M. Konikoff, “Gallstones – approach to medical management,” Med. Gen. Med., 5, No. 4, 8 (2003).Google Scholar
- 14.G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, London, (2007), 6th ed.Google Scholar