Assessment of the Efficacy of Nucleotide Therapy after Sciatic Nerve Injury
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Experimental and clinical data provide evidence of an increased need for pyrimidine nucleotides after peripheral nerve injury. The aim of the present work was to evaluate the neuroprotective efficacy of the agent Nucleo CMP (Ferrer International, which contains the nucleotides cytidine monophosphate and uridine triphosphate), after unilateral crushing of the sciatic nerve in rats. Extracellular spike activity was recorded from individual spinal cord motoneurons on the side ipsilateral to the injury using high-frequency stimulation of the distal branches (tibial nerve, common peroneal nerve) of the injured sciatic nerve. The extent and ratio of excitatory/inhibitory evoked motoneuron responses in the Nucleo CMP group approached normal. The Nucleo CMP group showed recovery of measures in the abduction reflex test and the static sciatic index of the injured limb by day 30. Single therapeutic doses of Nucleo CMP given to intact animals increased baseline and evoked spike activity of individual motoneurons from 10 to 80 min.
Keywordssciatic nerve crushing Nucleo CMP spinal cord motoneurons
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