Neuroscience and Behavioral Physiology

, Volume 39, Issue 2, pp 207–210 | Cite as

Involvement of the sacral parasympathetic nucleus in the innervation of the descending colon and rectum in cats

  • A. A. Dorofeeva
  • S. S. Panteleev
  • F. N. Makarov


Studies in anesthetized (urethane, 1.5 g/kg, i.p.) cats using retrograde transport of horseradish peroxidase addressed the locations and morphometric characteristics of neurons in the sacral parasympathetic nucleus of the spinal cord innervating the descending colon and rectum. Marker solution was injected beneath the serous membrane of the study areas of the large intestine. Transcardiac perfusion with fixative solution was performed 48 h later and frontal sections of the sacral segments of the spinal cord were prepared; these were processed by the Mesulam method (1978). The results showed that these areas of the large intestine receive innervation from neurons in the sacral parasympathetic nucleus located in spinal cord segments SI, SII, and SIII. The largest number of labeled cells was seen in segment SII. The neurons of this nucleus innervating the study areas of the large intestine formed two longitudinally distributed group (a lateral and a dorsal), the cells of which differed in terms of size and the orientation of the long axis. The largest number of labeled cells was seen in the lateral group.

Key Words

large intestine sacral parasympathetic nucleus horseradish peroxidase 


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  1. 1.
    A. A. Dorofeeva and S. S. Panteleev, “Intestinal motor responses to stimulation of sacral parasympathetic nucleus neurons,” Ros. Fiziol. Zh. im. I. M. Sechenova, 93, No. 10, 1188–1195 (2007).Google Scholar
  2. 2.
    A. A. Dorofeeva, S. S. Panteleev, and F. N. Makarov, “Parasympathetic innervation of the initial segments of the large intestine in cats,” Morfologiya, 132, No. 6, 34–39 (2007).Google Scholar
  3. 3.
    A. A. Dorofeeva, S. S. Panteleev, L. A. Markova, et al., “Topical organization of neurons in spinal cord ganglia innervating the large intestine,” Morfologiya, 130, No. 6, 47–50 (2006).Google Scholar
  4. 4.
    A. Rakhishev Morphology of the Pelvic Plexus [in Russian], Alma-Ata, Kazakhstan (1971).Google Scholar
  5. 5.
    B. Banrezes, P. Andrey, E. Maschino, et al, “Spatial segregation within the sacral parasympathetic nucleus of neurons innervating the bladder or the penis of the rat as revealed by three-dimensional reconstruction, ” Neurosci., 115, No. 1, 97–109 (2002).CrossRefGoogle Scholar
  6. 6.
    H. R. Berthoud, N. R. Carlson, and T. L. Powley, “Topography of efferent vagal innervation of the rat gastrointestinal tract,” Amer. J. Physiol., 260, No. 1, Part 2, 200–207 (1991).Google Scholar
  7. 7.
    W. C. de Groat, A. M. Booth, R. J. Milne, and J. R. Roppolo, “Parasympathetic preganglionic neurons in the sacral spinal cord,” J. Auton. Nerv. Syst., 5, No. 1, 23–43 (1982).PubMedCrossRefGoogle Scholar
  8. 8.
    W. C. de Groat, I. Nadelhaft, R. J. Milne, et al., “Organization of the sacral parasympathetic reflex pathways to the urinary bladder and large intestine,” J. Auton. Nerv syst., 3, 135–160 (1981).PubMedCrossRefGoogle Scholar
  9. 9.
    W. Jänig and E. M. McLachlan, “Organization of lumbar spinal outflow to distal colon and pelvic organs,” Physiol. Rev., 67, No. 4, 1332–1404 (1987).PubMedGoogle Scholar
  10. 10.
    M.-M. Mesulam, “Tetramethylbenzidine for horseradish per-oxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents,” J. Histochem. Cytochem., 26, No. 2, 106–117 (1978).PubMedGoogle Scholar
  11. 11.
    C. Morgan, I. Nadelhaft, and W. C. de Groat, “Location of bladder preganglionic neurons within the sacral parasympathetic nucleus of the cat,” Neurosci. Lett., 14, No. 2–3, 189–194 (1979).PubMedCrossRefGoogle Scholar
  12. 12.
    I. Nadelhaft, W. C. de Groat, and C. Morgan, “Location and morphology of parasympathetic preganglionic neurons in the sacral spinal cord of the cat revealed by retrograde axonal transport of horseradish peroxidase,” J. Comp. Neurol., 193, No. 1, 265–281 (1980).PubMedCrossRefGoogle Scholar
  13. 13.
    R. E. Papka, J. R. McCurdy, S. J. Williams, et al., “Parasympathetic preganglionic neurons in the spinal cord involved in uterine innervation are cholinergic and nitric oxide-containing,” Anat. Rec., 241, No. 4, 554–562 (1995).PubMedCrossRefGoogle Scholar
  14. 14.
    H. Satomi, T. Yamamoto, H. Ise, and H. Takatama, “Origins of the parasympathetic fibers to the cat intestine as demonstrated by the horseradish peroxidase method,” Brain Res., 151, No. 3, 571–578 (1978).PubMedCrossRefGoogle Scholar
  15. 15.
    C. Tai, A. M. Booth, W. C. de Groat, and J. R. Roppolo, “Colon and anal sphincter contractions evoked by microstimulation of the sacral spinal cord in cats,” Brain Res., 889, No. 1–2, 38–48 (2001).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • A. A. Dorofeeva
    • 1
  • S. S. Panteleev
    • 1
  • F. N. Makarov
    • 2
  1. 1.Corticovisceral Physiology Laboratory (Director: Doctor of Biological Sciences S. S. Panteleev)St. PetersburgRussia
  2. 2.Neuromorphology Laboratory (Director: Doctor of Medical Sciences F. N. Makarov), I. P. Pavlov Institute of PhysiologyRussian Academy of SciencesSt. PetersburgRussia

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