Age-related characteristics of the neurotransmitter composition of neurons in the stellate ganglion
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Abstract
The neurotransmitter composition of neurons in the stellate ganglion of rats of different ages (neonatal, 10, 20, 30, and 60 days) was studied by an immunocytochemical method using double labeling. Most neurons in rat pups of all age groups contained tyrosine hydroxylase. Most choline acetyltransferase-positive neurocytes in neonatal and 10-day-old rat pups were also tyrosine hydroxylase-positive. Only occasional cells in 30-and 60-day rat pups contained both of these enzymes. There were increases in the proportions of cells containing tyrosine hydroxylase and neuropeptide Y from birth to all time points of the study. In addition, there was a decrease in the proportion of somatostatin-positive neurons. The proportions of VIP-positive cells and choline acetyltransferase-containing neurons increased to age 10 days and then decreased. Somatostatin-positive neurons in all rat pups were small cells, while those containing choline acetyltransferase were large. Maturation of the neurotransmitter set in the rat stellate ganglion was complete by the end of the second month of life.
Key words
sympathetic nervous system stellate ganglion immunocytochemistry ontogenesisPreview
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References
- 1.A. D. Nozdrachev, “The chemical structure of a peripheral autonomic (visceral) reflex,” Usp. Fiziol. Nauk., 27, No. 2, 28–60 (1996).PubMedGoogle Scholar
- 2.A. D. Nozdrachev and A. V. Yantsev, Autonomic Transmission [in Russian], St. Petersburg (1995).Google Scholar
- 3.A. D. Nozdrachev and M. M. Fateev, The Stellate Ganglion. Structure and Function [in Russian], St. Petersburg (2002).Google Scholar
- 4.V. S. Sheveleva, Evolution of the Functions of the Sympathetic Ganglia in Ontogenesis [in Russian], Leningrad (1977).Google Scholar
- 5.A. G. M. Bullock, “Somatostatin enhances neurite outgrowth and electrical coupling of regenerating neurons in Helisoma,” Brain Res., 412, 6–17 (1987).CrossRefGoogle Scholar
- 6.P. Cochard, M. Goldstein, and I. B. Black, “Initial development of the noradrenergic phenotype in autonomic neuroblasts of the rat embryo in vivo,” Dev. Biol., 71, 109–114 (1979).CrossRefGoogle Scholar
- 7.H. H. Dale and W. Feldberg, “The chemical transmission of secretory impulses to the sweat glands of the cat,” J. Physiol., 82, 121–128 (1934).PubMedGoogle Scholar
- 8.U. Ernsberger, “The development of postganglionic sympathetic neurons: coordinating neuronal differentiation and diversification,” Auton. Neurosci. Basic Clin., 94, 1–13 (2001).CrossRefGoogle Scholar
- 9.U. Ernsberger and H. Rohrer, “Development of the cholinergic neurotransmitter phenotype in postganglionic sympathetic neurons,” Cell Tiss. Res., 297, 339–361 (1999).CrossRefGoogle Scholar
- 10.U. S. von Euler, “A specific sympathomimetic ergone in sympathetic nerve fibers (sympathin) and its relation to adrenaline and noradrenaline,” Acta Physiol. Scand., 12, 73–97 (1946).Google Scholar
- 11.J. B. Furness, J. L. Morris, I. L. Gibbins, and M. Costa, “Chemical coding of neurons and plurichemical transmission,” Ann. Rev. Pharmacol. Toxicol., 29, 289–306 (1989).CrossRefGoogle Scholar
- 12.I. L. Gibbins, “Vasocomotor, pilomotor and secretomotor neurons distinguished by size and neuropeptide content in superior cervical ganglia of mice,” J. Auton. Nerve Syst., 34, 171–183 (1991).CrossRefGoogle Scholar
- 13.L. Klimaschewski, W. Kummer, and C. Heym, “Localization, regulation and function of neurotransmitters and neuromodulators in cervical sympathetic ganglia,” Microsc. Res. Tech., 35, 44–68 (1996).PubMedCrossRefGoogle Scholar
- 14.P. M. Masliukov, V. A. Pankov, A. A. Strelkov, E. A. Masliukova, V. V. Shilkin, and A. D. Nozdrachev, “Morphological features of neurons innervating different viscera in the cat stellate ganglion in postnatal ontogenesis,” Aut. Neurosci. Basic Clin., 84, 169–175 (2000).CrossRefGoogle Scholar
- 15.P. M. Masliukov, V. V. Shilkin, A. D. Nozdrachev, and J.-P. Timmermans, “Histochemical features of neurons in the cat stellate ganglion during postnatal ontogenesis,” Aut. Neurosci. Basic Clin., 106, 84–90 (2003).CrossRefGoogle Scholar
- 16.M. A. Morales, K. Holmberg, Z. Q. Xu, C. Cozzari, B. K. Hartman, P. Emson, M. Goldstein, L. G. Elfvin, and T. Hokfelt, “Localization of choline acetyltransferase in rat peripheral sympathetic neurons and its coexistence with nitric oxide synthase and neuropeptides,” Proc. Natl. Acad. Sci. USA, 92, 11819–11823 (1995).Google Scholar
- 17.D. W. Pincus, E. M. DiCicco-Bloom, and I. B. Black, “Vasoactive intestinal peptide regulates mitosis, differentiation and survival of cultured sympathetic neuroblasts,” Nature, 343, 564–567 (1990).PubMedCrossRefGoogle Scholar
- 18.V. Roudenok, “Changes in the expression of neuropeptide Y (NPY) during maturation of human sympathetic ganglionic neurons: correlations with tyrosine hydroxylase immunoreactivity,” Ann. Anat., 182, 515–519 (2000).PubMedCrossRefGoogle Scholar
- 19.H. M. Young, R. B. Anderson, and C. R. Anderson, “Guidance cues involved in the development of the peripheral autonomic nervous system,” Aut. Neurosci. Basic Clin., 112, 1–14 (2004).CrossRefGoogle Scholar