Neuroscience and Behavioral Physiology

, Volume 37, Issue 4, pp 341–347 | Cite as

Transcranial electrostimulation activates reparative regeneration and the insulin-producing function of pancreatic B-cells in alloxan diabetes in rats

  • V. P. Lebedev
  • S. V. Bilichenko
  • N. É. Ordyan
  • S. G. Pivina
  • S. P. Nechiporenko
  • A. A. Puzyrev
  • E. A. Mikheeva
  • K. K. Kubacheva


Studies on rats with experimental diabetes induced by administration of alloxan showed that transcranial electrostimulation of endorphinergic brain structures stimulates the regeneration of damaged β-cells in pancreatic islets of Langerhans. This was identified on pancreatic sections stained with hematoxylin and eosin. De novo formation of small islets was noted, as evidenced by their regeneration from progenitor cells. After transcranial electrostimulation, islet β-cells stained by the Gomori method showed recovery of granularity-a sign of insulin production. Application of an immunoenzyme method demonstrated recovery of blood insulin levels, the dynamics of increases in which showed a highly significant negative correlation with a decrease in blood glucose. These data led to the conclusion that the antihyperglycemic effect of transcranial electrostimulation in experimental alloxan diabetes results from reparative regeneration of β-cells in islets of Langerhans with recovery of their insulin-producing function.

Key words

alloxan diabetes transcranial electrostimulation antihyperglycemic effect islet of Langerhans β-cells reparative regeneration insulin production 


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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • V. P. Lebedev
    • 1
  • S. V. Bilichenko
    • 1
    • 2
  • N. É. Ordyan
    • 1
  • S. G. Pivina
    • 1
  • S. P. Nechiporenko
    • 2
  • A. A. Puzyrev
    • 3
  • E. A. Mikheeva
    • 3
  • K. K. Kubacheva
    • 4
  1. 1.I. P. Pavlov Institute of PhysiologyRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Institute of ToxicologyMinistry of Health of the Russian FederationSt. PetersburgRussia
  3. 3.I. I. Mechnikov State Medical AcademySt. PetersburgRussia
  4. 4.Postgraduate Medical AcademySt. PetersburgRussia

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