Immunocytochemical detection of brain neurons using the selective marker NeuN
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Abstract
The aim of the present work was to develop optimal protocols for immunocytochemical reactions for nuclear protein NeuN for light and laser confocal microscopy which avoid the thermal antigen demasking procedure, which degrades the state of the tissue and requires use of expensive adhesive-coated slices. Maximal antigen retention was obtained after fixation in zinc-formalin and Bouin’s fluid (maximum one day). Two protocols are proposed allowing the thermal demasking procedure to be avoided during detection of neuron marker NeuN on paraffin sections examined by light and confocal microscopy.
Key words
brain neurons nuclear protein NeuN immunocytochemistry confocal microscopyPreview
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References
- 1.D. É. Korzhevskii, “The use of monoclonal antibodies to the nucleus protein PCNA to detect proliferating cells in the developing human brain,” Morfologiya, 118, No. 5, 68–70 (2000).Google Scholar
- 2.R. Gittins and P. J. Harrison, “Neuronal density, size and shape in the human anterior cingulate cortex: a comparison of Nissl and NeuN staining,” Brain Res. Bull., 63, No. 2, 155–160 (2004).PubMedCrossRefGoogle Scholar
- 3.D. C. Hess, W. Hill, A. Martin-Stoddard, et al., “Bone marrow as a source of endothelial cells and NeuN-expressing cells after stroke,” Stroke, 33, No. 5, 1362–1368 (2002).PubMedCrossRefGoogle Scholar
- 4.S. J. Naish, Immunochemical Staining Methods, DAKO Corporation (1989).Google Scholar
- 5.H. B. Sarnat, D. Nochlin, and D. E. Born, “Neuronal nuclear antigen (NeuN): a marker of neuronal maturation in early human fetal nervous system,” Brain Dev., 20, No. 2, 88–94 (1998).PubMedCrossRefGoogle Scholar
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© Springer Science+Business Media, Inc. 2006