Formation of Behavioral Pathology in Female C57BL/6J Mice Exposed to Prolonged Negative Psychoemotional Conditions
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Prolonged exposure of females to negative psychoemotional conditions by permanent keeping with aggressive males on the other side of a perforated partition in a cage with daily witnessing of intermale confrontations had negative influences on the behavior of females, assessed using a variety of ethological tests. The elevated maze test and the open field test provided evidence for the development of marked anxiety in the females, with inhibition of motor and investigative activity. Decreases in the responses of females close to the partition to familiar and unfamiliar males one month after the onset of exposure to negative psychoemotional conditions and before the end of the experiment provided evidence for a decrease in communicativeness. After two months of exposure to negative psychoemotional conditions, intermale confrontations also ceased to interest females, as shown by the partition test. The hotplate test revealed worsening of pain sensitivity in experimental females. More than half of the individuals demonstrated impairment to the cyclicity of sexual cycles with increases in the duration of diestrus. After being kept in negative psychoemotional conditions, females ceased to differentiate littermates by social status and gender, as shown by social/sexual recognition tests. All these data provide grounds for suggesting the development of an anxious-depressive state in females after being kept in negative psychoemotional conditions for prolonged periods.
Key WordsC57BL/6J mice negative psychoemotional conditions anxiety social recognition pain sensitivity communicativeness estral cycle anxious-depressive state
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- 1.D. F. Avgustinovich, “Anxiety in females induced by prolonged exposure to negative psychoemotional conditions,” Ros. Fiziol. Zh. im. I. M. Sechenova, 89, No.7, 858–867 (2003).Google Scholar
- 2.D. F. Avgustinovich, N. A. Lyudvina, V. V. Dolgov, and O. V. Alekseenko, “Effects of prolonged restriction stress on the behavior of female mice of the inbred strains C57BL/6J and CBA/Lac. Dispersion and cluster analysis,” Ros. Fiziol. Zh. im. I. M. Sechenova, 87, No.9, 1268–1282 (2001).Google Scholar
- 3.D. F. Avgustinovich and I. L. Kovalenko, “Prolonged individual keeping of C57BL/6J mice leads to anxiety, ” Ros. Fiziol. Zh. im. I. M. Sechenova, 90, No.3, 1268–1282 (2004).Google Scholar
- 4.E. G. Filatova and A. M. Vein, “Motivational and endocrine disturbances in depression,” Psikhiatr. Psikhofarmakoterapiya, 1 (2000).Google Scholar
- 25.R. Lister, “The use of a plus-maze to measure anxiety in the mouse,” Psychopharmacol., 92, 180–195 (1987).Google Scholar
- 26.D. H. Overstreet, O. Pucilowski, M. C. Retton, P. Delagrange, and B. Guardiola-Lemaitre, “Effects of melatonin receptor ligands on swim test immobility,” 9, 249–253 (1998).Google Scholar
- 29.R. D. Porsholt, A. Bertin, and M. Jalfre, “Behavioral despair in mice: A Primary screening test for antidepressants,” Arch. Int. Pharmacodynamics, 229, 327–336 (1977).Google Scholar
- 31.R. J. Rogers and C. Cole, “The elevated plus-maze: pharmacology, methodology and ethology,” in: Ethology and Psychopharmacology, S. J. Cooper and C. A. Hendrie (eds.), John Wiley & Sons Ltd., Chichester (1994), pp. 9–44.Google Scholar
- 37.J. M. Weiss, P. A. Goodman, B. G. Losito, S. Corrigans, J. M. Charry, and W. H. Bailey, “Behavioral depression produced by an uncontrollable stressor: relationship to norepinephrine, dopamine, and serotonin levels in various regions of rat brain,” Brain Res. Rev., 3, 167–205 (1981).CrossRefGoogle Scholar