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Chitosan-coated rectangular DNA nanospheres for better outcomes of anti-diabetic drug

  • Mirza Muhammad Faran Ashraf BaigEmail author
  • Muhammad Naveed
  • Muhammad Abbas
  • Said Abasse Kassim
  • Ghulam Jilany Khan
  • Sana Ullah
  • Muhammad Sohail
  • Waqas Nawaz
  • Muhammad Rizwan Younis
  • Muhammad Tayyab Ansari
Research Paper
Part of the following topical collections:
  1. Nanoparticles in Biotechnology and Medicine

Abstract

Type 2 diabetes is a metabolic disorder in which the patient fails to control the glycemic level due to compromised functioning of pancreatic α and β cells. The control of these cells is regulated by the extent of incretin peptides (GLP1 and GIP). Performance of these peptides is affected due to the increased degradation by di-peptidyl-peptidase-4 (DPP-4) enzyme in diabetic patients. Vildagliptin (VL) is one of the potential DPP-4 inhibitors and helps in controlling the glycemic level, but to deliver the VL near its site of action (intestine and blood) in a sustained manner is important. For this purpose, we used chitosan (Chit) (cationic polymer) to coat the VL-loaded DNA rectangles (negatively charged) via electrostatic attractions to make Chit-DNA-VL nanospheres. According to our results, nanospheres formulated by this novel approach were uniformed, spherical, and stable with better size control (from 40 to 400 nm in diameter). Entrapment efficiency for VL drug was up to 85%. The release of VL was extended up to 12 ± 5 h. From observed incretin and glycemic level, we concluded that the nanospheres efficiently bypassed the gastric acidity improving glycemic control in Db-Db/mouse model. Histological experiments revealed Chit-DNA-VL nanospheres did not cause damage to pancreas associated with the sustained and prolonged release of VL.

Keywords

Type 2 diabetes DNA nanospheres Vildagliptin Incretins Db-Db/mouse Nanomedicine 

Notes

Acknowledgments

We highly acknowledge Prof. Xing-Hua Xia of the State Key Laboratory of Analytical Chemistry for Life Sciences, Nanjing University, for the support. We are thankful to Wen Chunxia and Muhammad Hasnat for assisting us during the work. We acknowledge Maryam Sharif, Production Officer, High-Q/Pharma Pvt. Ltd., Pakistan, for donating us pure VL and polymers for this work. Special thanks to Dr. Chen Yalan of Nanjing University for the support.

Author individual contributions

MMFAB designed the study, performed most of the experiments, and prepared the manuscript. MN, MA, SAK, SU, MS, and WS assisted the work. GJK, MRY, and MTA gave expert opinions about the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11051_2019_4534_MOESM1_ESM.docx (15 kb)
ESM 1 (DOCX 15 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Mirza Muhammad Faran Ashraf Baig
    • 1
    • 2
    Email author
  • Muhammad Naveed
    • 3
  • Muhammad Abbas
    • 4
  • Said Abasse Kassim
    • 5
  • Ghulam Jilany Khan
    • 6
    • 7
  • Sana Ullah
    • 4
  • Muhammad Sohail
    • 3
  • Waqas Nawaz
    • 8
  • Muhammad Rizwan Younis
    • 1
  • Muhammad Tayyab Ansari
    • 2
  1. 1.State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical EngineeringNanjing UniversityNanjingPeople’s Republic of China
  2. 2.Department of Pharmaceutical Chemistry, Faculty of PharmacyBahauddin Zakariya UniversityMultanPakistan
  3. 3.School of Pharmacy, Department of Clinical PharmacologyNanjing Medical UniversityNanjingPeople’s Republic of China
  4. 4.School of Life Sciences, State Key Laboratory of Pharmaceutical BiotechnologyNanjing UniversityNanjingPeople’s Republic of China
  5. 5.Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public HealthSoutheast UniversityNanjingPeople’s Republic of China
  6. 6.Faculty of PharmacyUniversity of Central PunjabLahorePakistan
  7. 7.Jiangsu Key Laboratory of Drug ScreeningChina Pharmaceutical UniversityNanjingPeople’s Republic of China
  8. 8.Center for Public Health ResearchMedical School of Nanjing UniversityNanjingPeople’s Republic of China

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