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Particle Background Levels In Human Tissues—PABALIHT project. Part I: a nanometallomic study of metal-based micro- and nanoparticles in liver and kidney in an Italian population group

  • Emanuela Locci
  • Ilaria PiliaEmail author
  • Roberto Piras
  • Sergio Pili
  • Gabriele Marcias
  • Pierluigi Cocco
  • Fabio De Giorgio
  • Manuele Bernabei
  • Valentina Brusadin
  • Laura Allegrucci
  • Alessandra Bandiera
  • Ernesto d’Aloja
  • Enrico Sabbioni
  • Marcello Campagna
Research Paper
  • 77 Downloads

Abstract

We used field-emission scanning electron microscopy (FESEM) coupled with energy dispersive X-ray spectroscopy (EDS) microanalysis to determine size, shape, and elemental composition of particles in the liver and kidney autopsy of 35 subjects living in Sardinia. Particles were detected in 94% of livers and in 97% of kidney samples as aggregates, ranging between 50 nm and 100 μm. A total of 513 particle aggregates were observed in all tissues (276 in the liver and 237 in the kidney samples). Aggregates consisted of single elements (As, Ba, Cu, F, Fe, Ge, Hg, Ni, P, Pb, S, Si, Ti) or multiple association of elements, up to six. Silicon and Fe were the main element constituents (presence in 61% and 23% of the total Pags) followed by Al, Hg, Ni, Pb, and Ti (< 1%). Calcium, Cl, Co, Cr, Na, K, P, Sc, Sn, W, V, Zn, sometimes Mg, Rh, and Ta, were also detected. Overall, our findings suggest that the ubiquitous presence of metal species as particle aggregates in human tissues would be a condition of normality, although such presence per se is far from being used as a toxicity biomarker. Before being able to clarify the implications of particles in diseases or normal physiological processes, the biological meaning of particles background level we observed in liver and kidney must be elucidated.

Keywords

Particles kinetic Nanoparticle Human tissues General population Field-emission scanning electron microscopy Biomedicine Environmental and health effects 

Abbreviations

Ps

Particles

FESEM

Field-emission scanning microscopy

EDS

Energy dispersive X-ray spectroscopy

Pag

Particle aggregate

MeNPs

Metal-based nanoparticles

PABALIHT

Particle Background Levels In Human Tissues

ENPs

Engineered nanoparticles

WD

Work distance

SDD

Silicon drift detector

AuNPs

Gold nanoparticles

AgNPs

Silver nanoparticles

NaAuCl4·2H2O

Sodium tetrachloroaurate (III)dehydrate

AgNO3

Silver nitrate

SD

Standard deviation

NPag

Nanoparticle aggregates

MPag

Micro-particle aggregates

NPs

Nanoparticles

MPS

Mononuclear phagocytic system

Notes

Acknowledgements

This study marks the beginning of the PABALHIT project. Each department is contributing to the project with its own research funds. The authors are grateful to Prof Cesare Castellini (Department of Applied Biology, University of Perugia, Italy) who made possible our study on leaching with formalin/ethanol on rabbit liver and kidney. Such tissues were obtained in the context of a research program he carried out on the effects of nanoparticles on the reproductive activity of buck rabbits as conducted in accordance with the Guiding Principles on Animal Use in Toxicology and approved by the Animal Ethics Monitoring Committee at the University of Siena.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Emanuela Locci
    • 1
  • Ilaria Pilia
    • 1
    Email author
  • Roberto Piras
    • 1
  • Sergio Pili
    • 1
  • Gabriele Marcias
    • 1
  • Pierluigi Cocco
    • 1
  • Fabio De Giorgio
    • 2
  • Manuele Bernabei
    • 3
  • Valentina Brusadin
    • 3
  • Laura Allegrucci
    • 3
  • Alessandra Bandiera
    • 3
  • Ernesto d’Aloja
    • 1
  • Enrico Sabbioni
    • 4
    • 5
  • Marcello Campagna
    • 1
  1. 1.Department of Medical Sciences and Public HealthUniversity of CagliariCagliariItaly
  2. 2.Institute of Public Health, School of MedicineCatholic UniversityRomeItaly
  3. 3.Chemical Department, Experimental Flight CentreAirport “M. De Bernardi”, Pratica di Mare AFBRomeItaly
  4. 4.CeSI-Aging Research CenterG. d’Annunzio University FoundationChietiItaly
  5. 5.LASAUniversity of Studies of Milan and INFN-MilanMilanItaly

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