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Detection and analysis of human serum albumin nanoparticles within phagocytic cells at the resolution of individual live cell or single 3D multicellular spheroid

  • Elena Afrimzon
  • Naomi Zurgil
  • Maria Sobolev
  • Yana Shafran
  • Klaus Langer
  • Iavor Zlatev
  • Robert Wronski
  • Manfred Windisch
  • Hagen von Briesen
  • Reinhold Schmidt
  • Claus Pietrzik
  • Mordechai DeutschEmail author
Research Paper
  • 178 Downloads

Abstract

Since nanoparticles (NPs) have shown great potential in various biomedical applications, live cell response to NPs should be thoroughly explored prior to their in vivo use. In the current study, live cell array (LCA) methodology and unique cell-based assays were used to study the interaction of magnetite (HSA-Mag NP) loaded human serum albumin NPs with phagocytic cells. The LCA enabled cell culturing during HSA-Mag NP accumulation and monolayer or spheroid formation, concomitantly with on-line monitoring of NP internalization. These platforms were also utilized for imaging intercellular links between living cells preloaded with HSA-Mag NP in 2D and 3D resolution. HSA-Mag NP uptake by cells was quantified by imaging, and analyzed using time-resolved measurements. Image analysis of the individual cells in cell populations showed accumulation of HSA-Mag NP by promonocytes and glial cells in a dose- and time-dependent manner. High variability of NP accumulation in individual cells within cell populations, as well as cell subgroups, was evident in both cell types. Following 24 h interaction, uptake of HSA-Mag NP was about 10 times more efficient in glial cells than in activated promonocytes. The presented assays may facilitate detection and analysis of the amount of NPs within individual cells, as well as the rate of NP accumulation and processing in different subsets of living cells. Such data are crucial for estimating predicted drug dosage delivered by NPs, as well as to study possible mechanisms for NP interference with live cells.

Keywords

Human serum albumin NPs Phagocytic cells Single cell/spheroid Image analysis Targeted drug delivery Nanomedicine 

Notes

Acknowledgments

This study was endowed by the Bequest of Moshe-Shimon and Judith Weisbrodt, by the ERA-NET NEURON Transnational Research Project 2009 and by the Israel Ministry of Health.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Standards

This manuscript does not contain clinical studies or patient data.

Supplementary material

Supplementary material 1 (MP4 1498 kb)

Supplementary material 2 (MP4 593 kb)

11051_2015_3306_MOESM3_ESM.docx (277 kb)
Supplementary material 3 (DOCX 276 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Elena Afrimzon
    • 1
  • Naomi Zurgil
    • 1
  • Maria Sobolev
    • 1
  • Yana Shafran
    • 1
  • Klaus Langer
    • 2
  • Iavor Zlatev
    • 2
  • Robert Wronski
    • 3
  • Manfred Windisch
    • 3
  • Hagen von Briesen
    • 4
  • Reinhold Schmidt
    • 5
  • Claus Pietrzik
    • 6
  • Mordechai Deutsch
    • 1
    Email author
  1. 1.The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the CellomeBar-Ilan UniversityRamat GanIsrael
  2. 2.Institut für Pharmazeutische Technologie und BiopharmazieWestfälischen Wilhelms-Universität MünsterMünsterGermany
  3. 3.QPS Austria GmbHGrambachAustria
  4. 4.Department of Cell Biology & Applied VirologyFraunhofer Institute for Biomedical Engineering IBMTSt. IngbertGermany
  5. 5.Department of NeurologyMedical University of GrazGrazAustria
  6. 6.Institute of PathobiochemistryUniversity Medical Center of the Johannes Gutenberg University of MainzMainzGermany

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