Impact of surface coating and food-mimicking media on nanosilver-protein interaction
- 352 Downloads
The application of silver nanoparticles (AgNPs) in food contact materials has recently become a subject of dispute due to the possible migration of silver in nanoform into foods and beverages. Therefore, the analysis of the interaction of AgNPs with food components, especially proteins, is of high importance in order to increase our knowledge of the behavior of nanoparticles in food matrices. AgPURE™ W10 (20 nm), an industrially applied nanomaterial, was compared with AgNPs of similar size frequently investigated for scientific purposes differing in the surface capping agent (spherical AgNP coated with either PVP or citrate). The interactions of the AgNPs with whey proteins (BSA, α-lactalbumin and β-lactoglobulin) at different pH values (4.2, 7 or 7.4) were investigated using surface plasmon resonance, SDS-PAGE, and asymmetric flow field-flow fractionation. The data obtained by the three different methods correlated well. Besides the nature of the protein and the nanoparticle coating, the environment was shown to affect the interaction significantly. The strongest interaction was obtained with BSA and AgNPs in an acidic environment. Neutral and slightly alkaline conditions however, seemed to prevent the AgNP-protein interaction almost completely. Furthermore, the interaction of whey proteins with AgPURE™ W10 was found to be weaker compared to the interaction with the other two AgNPs under all conditions investigated.
KeywordsSilver nanoparticles Whey proteins BSA Beta-lactoglobulin Alpha-lactalbumin Protein corona
The authors would like to thank A. Lauckner-Tessin, I. Ebert, A. Tauer, and F. Mohr for their excellent technical assistance, Dr. D. Behsnilian for SEM analysis, and Dr. K. Oehlke for valuable discussions during preparation of the manuscript.
Compliance with ethical standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Bolea E, Jimenez-Lamana J, Laborda F, Abad-Alvaro I, Blade C, Arola L, Castillo JR (2014) Detection and characterization of silver nanoparticles and dissolved species of silver in culture medium and cells by asflfff-uv-vis-icpms: application to nanotoxicity tests. Anal 139:914–922CrossRefGoogle Scholar
- Klein CL et al. (2011) Nm-300 silver characterisation, stability, homogeneity. Publications Office of the European Union EUR 24693 EN:1-84Google Scholar
- Podila R, Chen R, Ke PC, Brown JM, Rao AM (2012) Effects of surface functional groups on the formation of nanoparticle-protein corona. Appl Phys Lett 101:263701–263701–263704Google Scholar
- RIKILT, JRC (2014) Inventory of nanotechnology applications in the agricultural, feed and food sector. EFSA supporting publication, EN-621:1–125Google Scholar
- Uvex-safety http://www.Uvex-safety.com/en/products/protective-clothing/disposable-coveralls/technology-disposable-coveralls. Accessed 02 April 2015