Targeting and molecular imaging of HepG2 cells using surface-functionalized gold nanoparticles
- 353 Downloads
Mercaptosuccinic acid (M)-conjugated gold nanoparticles (GM) were prepared and characterized by transmission electron microscope and dynamic light scattering. M was used to improve the monodispersity and non-specific intracellular uptake of nanoparticles. Lactobionic acid (L) was subsequently conjugated to the GM to target preferentially HepG2 cells (liver cancer cells) that express asialoglycoprotein receptors (ASGPR) on their membrane surfaces and facilitate the transit of nanoparticles across the cell membrane. The mean size of lactobionic acid-conjugated gold nanoparticle (GL) was approximately 10 ± 0.2 nm. Finally, the Atto 680 dye (A6) was coupled to the nanoparticles to visualize their internalization into HepG2 cells. The interaction of surface-modified gold nanoparticles with HepG2 cells was studied after culturing cells in media containing the GM or L-conjugated GM (GL).
KeywordsLactobionic acid Gold nanoparticles HepG2 cells Intracellular uptake Nanomedicine
This study was supported by the Basic Research Laboratory Program (No. 2011-0020264) and the General Research Program (2013 R1A1A 2005148) from the Ministry of Education, Science and Technology of Korea.
- Allen TM (2002) Ligand-targeted therapeutics in anticancer therapy. Angew Chem Int Ed 2(1):705–763Google Scholar
- Crespo P (2004) Permanent magnetism, magnetic anisotropy, and hysteresis of thiol-capped gold nanoparticles. Phys Rev Lett 93(1):872041–872044Google Scholar
- Gan J, Jiang S, Yang Y (2008) Immobilization of homing peptide on magnetite nanoparticles and its specificity in vitro. J Biomed Mater Res A 27(1):468–476Google Scholar
- Geelan T, Nicoloy K, Paulis LEM, Yeo SY, Strijkers GJ (2012) Internalization of paramagnetic phosphatidylserine-containing liposomes by macrophages. J Nano Biotechnol 10(37):1–11Google Scholar
- Mannervik B (1987) The enzymes of glutathione metabolism: an overview. Biochem Soc Trans 15(1):717–718Google Scholar