Synthesis and characterization of silver colloidal nanoparticles with different coatings for SERS application

  • L. Mikac
  • M. Ivanda
  • M. Gotić
  • T. Mihelj
  • L. Horvat
Research Paper


Silver colloids were produced by chemical reduction of silver salt (silver nitrate, AgNO3) solution. As reducing agents, trisodium citrate, sodium borohydride, ascorbic acid, polyvinylpyrrolidone, and glucose were used. The colloids were characterized by UV–Vis, DLS, zeta potential measurements, and SEM. The colloids were stabilized with negative groups or large molecules attached to their surface. The surface-enhanced Raman scattering (SERS) effect of stabilized nanoparticles was measured by using pyridine and rhodamine 6G molecules as analytes and NaNO3, KCl, and KBr at different concentrations as aggregating agents. The best Raman signal enhancement was achieved using silver nanoparticles of 40 nm size reduced and stabilized with citrate. The SERS signal of analyte molecules was further enhanced with the addition of sodium borohydride as an alternative aggregating agent. The borohydride had the strongest impact on the SERS effect of the colloid consistent of large (0.5 µm) silver nanoparticles stabilized with aminodextran. The mixture colloid-borohydride-pyridine was stable for hours. The mechanism of borohydride in the colloids is discussed.


Silver nanoparticles Colloid Reducing agents Aggregating agents SERS Pyridine 



This work was supported by the Ministry of Science and Technology of the Republic of Croatia, Project Number 098-0982904- 2898. Financial support by the Croatian Center of Excellence for Advanced Materials and Sensors is gratefully acknowledged. This work was performed in the context of the European COST Action MP1302 Nanospectroscopy.

Supplementary material

11051_2014_2748_MOESM1_ESM.pdf (804 kb)
Supplementary material 1 (PDF 803 kb)


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Copyright information

© European Union 2014

Authors and Affiliations

  • L. Mikac
    • 1
  • M. Ivanda
    • 1
  • M. Gotić
    • 2
  • T. Mihelj
    • 3
  • L. Horvat
    • 4
  1. 1.Laboratory for Molecular PhysicsRuđer Bošković InstituteZagrebCroatia
  2. 2.Laboratory for Synthesis of New MaterialsRuđer Bošković InstituteZagrebCroatia
  3. 3.Laboratory for Synthesis and Processes of Self-assembling of Organic MoleculesRuđer Bošković InstituteZagrebCroatia
  4. 4.Laboratory for Electron MicroscopyRuđer Bošković InstituteZagrebCroatia

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