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Silica nanoparticles mediated neuronal cell death in corpus striatum of rat brain: implication of mitochondrial, endoplasmic reticulum and oxidative stress

  • Arshiya Parveen
  • Syed Husain Mustafa Rizvi
  • Farzana Mahdi
  • Sandeep Tripathi
  • Iqbal Ahmad
  • Rajendra K. Shukla
  • Vinay K. Khanna
  • Ranjana Singh
  • Devendra K. Patel
  • Abbas Ali MahdiEmail author
Research Paper

Abstract

Extensive uses of silica nanoparticles (SiNPs) in biomedical and industrial fields have increased the risk of exposure, resulting concerns about their safety. We focussed on some of the safety aspects by studying neurobehavioural impairment, oxidative stress (OS), neurochemical and ultrastructural changes in corpus striatum (CS) of male Wistar rats exposed to 80-nm SiNPs. Moreover, its role in inducing mitochondrial and endoplasmic reticulum (ER) stress-mediated neuronal apoptosis was also investigated. The results demonstrated impairment in neurobehavioural indices, and a significant increase in lipid peroxide levels (LPO), hydrogen peroxide (H2O2), superoxide (O2 -) and protein carbonyl content, whereas there was a significant decrease in the activities of the enzymes, manganese superoxide dismutase (Mn SOD), glutathione peroxidase (GPx), catalase (CAT) and reduced glutathione (GSH) content, suggesting impaired antioxidant defence system. Protein (cytochrome c, Bcl-2, Bax, p53, caspase-3, caspase 12 and CHOP/Gadd153) and mRNA (Bcl-2, Bax, p53 and CHOP/Gadd153, cytochrome c) expression studies of mitochondrial and ER stress-related apoptotic factors suggested that both the cell organelles were involved in OS-mediated apoptosis in treated rat brain CS. Moreover, electron microscopic studies clearly showed mitochondrial and ER dysfunction. In conclusion, the result of the study suggested that subchronic SiNPs’ exposure has the potential to alter the behavioural activity and also to bring about changes in biochemical, neurochemical and ultrastructural profiles in CS region of rat brain. Furthermore, we also report SiNPs-induced apoptosis in CS, through mitochondrial and ER stress-mediated signalling.

Keywords

Silica nanoparticles Corpus striatum Neuronal apoptosis Mitochondrial stress Endoplasmic reticulum stress Oxidative stress 

Notes

Acknowledgments

We gratefully acknowledge the financial support received from Council of Science & Technology U. P. for carrying out the study.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Arshiya Parveen
    • 1
  • Syed Husain Mustafa Rizvi
    • 1
  • Farzana Mahdi
    • 2
  • Sandeep Tripathi
    • 3
  • Iqbal Ahmad
    • 4
  • Rajendra K. Shukla
    • 5
  • Vinay K. Khanna
    • 5
  • Ranjana Singh
    • 1
  • Devendra K. Patel
    • 6
  • Abbas Ali Mahdi
    • 1
    Email author
  1. 1.Department of BiochemistryKing George’s Medical UniversityLucknowIndia
  2. 2.Department of BiochemistryEra’s Lucknow Medical College & HospitalLucknowIndia
  3. 3.Department of BiotechnologyInstitute of Engineering and TechnologyJaipurIndia
  4. 4.Fibre ToxicologyCSIR-Indian Institute of Toxicology ResearchLucknowIndia
  5. 5.Developmental ToxicologyCSIR-Indian Institute of Toxicology ResearchLucknowIndia
  6. 6.Analytical Chemistry DivisionCSIR-Indian Institute of Toxicology ResearchLucknowIndia

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