In situ functionalized fluorescent nanoparticles for efficient receptor coupling
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An oligoethylene glycol-based propargyl phosphonate was applied to functionalize the surface of LaPO4:Ce,Tb nanoparticles in situ during the particle synthesis. The application of the surface modification reagent did not alter either size (5–7 nm in diameter) or morphology of the nanocrystalline core, but provided efficient anchor groups for subsequent coupling of a carbohydrate model receptor under mild reaction conditions. The biofunctionalization efficiency was quantified by thermogravimetric analysis and confirmed by a photometric assay. A calculation-based estimation suggested an average number of about 20 biomarkers per nanoparticles and a surface density of about 1 marker per 6 nm−2.
KeywordsNanoparticle synthesis Click chemistry-based particle coupling Biofunctionalization Nanocrystals Diagnostics and therapy
Financial support for this work by the University of Malaya under research Grants RG026-09AFR, RP024-2012B, PS380-2010B, and RG264-13AFR is gratefully acknowledged.
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