Eco-friendly decoration of graphene oxide with biogenic silver nanoparticles: antibacterial and antibiofilm activity

  • Andreia Fonseca de Faria
  • Ana Carolina Mazarin de Moraes
  • Priscyla Daniely Marcato
  • Diego Stéfani Teodoro Martinez
  • Nelson Durán
  • Antônio Gomes Souza Filho
  • Adriano Brandelli
  • Oswaldo Luiz Alves
Research Paper

Abstract

This work reports on preparation, characterization, and antibacterial activity of graphene oxide (GO) decorated with biogenic silver nanoparticles (Bio-AgNPs) produced by the fungus Fusarium oxysporum. This nanocomposite (Bio-GOAg) was prepared by an ex situ process through the physical mixture of a GO dispersion with the previously prepared Bio-AgNPs. The adsorption of the Bio-AgNPs onto the GO sheets was confirmed by transmission electron microscopy. The average size of the Bio-AgNPs anchored onto the GO surface was found to be 3.5 nm. The antibacterial activity of the Bio-GOAg nanocomposite against Gram-positive and Gram-negative microorganisms was investigated and a very promising result was found for the Gram-negative strains. In addition, the Bio-GOAg nanocomposite displayed a very strong biocidal activity against the Salmonella typhimurium strain at a concentration of 2.0 μg/mL. The antibiofilm activity toward S. typhimurium adhered on stainless steel surfaces was also investigated. The results showed 100 % inhibition of the adhered cells after exposure to the Bio-GOAg nanocomposite for 1 h.

Keywords

Graphene oxide Biogenic silver nanoparticles Fusarium oxysporum Antibacterial activity Salmonella typhimurium Microbiology 

Notes

Acknowledgments

A.F.F., P.D.M., D.S.T.M., A.C.M.M., N.D., and O.L.A. acknowledge the financial support of São Paulo State Research Foundation (FAPESP), National Council for Technological and Scientific Development (CNPq), National Institute of Science, Technology, and Innovation in Complex Functional Materials (INCT-Inomat), Brazilian Nanotoxicology Network (Cigenanotox), and NanoBioss-SisNano (MCTI). A.G.S.F. acknowledges the financial support of INCT NanoBioSimes (MCT-CNPq), Capes-Nanobiotec network, and Funcap through the grant Pronex (PR 2-0054-00022.01.00/11). A.B. acknowledges the financial support of CNPq and Capes. The authors acknowledge Prof.Amauri Jardim de Paula for his contributions to the transmission electron microscopy (TEM) analysis.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Andreia Fonseca de Faria
    • 1
  • Ana Carolina Mazarin de Moraes
    • 1
  • Priscyla Daniely Marcato
    • 1
    • 2
  • Diego Stéfani Teodoro Martinez
    • 1
  • Nelson Durán
    • 1
    • 3
  • Antônio Gomes Souza Filho
    • 4
  • Adriano Brandelli
    • 5
  • Oswaldo Luiz Alves
    • 1
  1. 1.Instituto de QuímicaUniversidade Estadual de CampinasCampinasBrazil
  2. 2.School of Pharmaceutical Science of Ribeirão PretoUniversidade de São Paulo (USP)Ribeirão PretoBrazil
  3. 3.Center of Natural and Human SciencesUniversidade Federal do ABCSanto AndréBrazil
  4. 4.Departamento de FísicaUniversidade Federal do CearáFortalezaBrazil
  5. 5.Instituto de Ciência e Tecnologia de AlimentosUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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