Eco-friendly decoration of graphene oxide with biogenic silver nanoparticles: antibacterial and antibiofilm activity
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This work reports on preparation, characterization, and antibacterial activity of graphene oxide (GO) decorated with biogenic silver nanoparticles (Bio-AgNPs) produced by the fungus Fusarium oxysporum. This nanocomposite (Bio-GOAg) was prepared by an ex situ process through the physical mixture of a GO dispersion with the previously prepared Bio-AgNPs. The adsorption of the Bio-AgNPs onto the GO sheets was confirmed by transmission electron microscopy. The average size of the Bio-AgNPs anchored onto the GO surface was found to be 3.5 nm. The antibacterial activity of the Bio-GOAg nanocomposite against Gram-positive and Gram-negative microorganisms was investigated and a very promising result was found for the Gram-negative strains. In addition, the Bio-GOAg nanocomposite displayed a very strong biocidal activity against the Salmonella typhimurium strain at a concentration of 2.0 μg/mL. The antibiofilm activity toward S. typhimurium adhered on stainless steel surfaces was also investigated. The results showed 100 % inhibition of the adhered cells after exposure to the Bio-GOAg nanocomposite for 1 h.
KeywordsGraphene oxide Biogenic silver nanoparticles Fusarium oxysporum Antibacterial activity Salmonella typhimurium Microbiology
A.F.F., P.D.M., D.S.T.M., A.C.M.M., N.D., and O.L.A. acknowledge the financial support of São Paulo State Research Foundation (FAPESP), National Council for Technological and Scientific Development (CNPq), National Institute of Science, Technology, and Innovation in Complex Functional Materials (INCT-Inomat), Brazilian Nanotoxicology Network (Cigenanotox), and NanoBioss-SisNano (MCTI). A.G.S.F. acknowledges the financial support of INCT NanoBioSimes (MCT-CNPq), Capes-Nanobiotec network, and Funcap through the grant Pronex (PR 2-0054-00022.01.00/11). A.B. acknowledges the financial support of CNPq and Capes. The authors acknowledge Prof.Amauri Jardim de Paula for his contributions to the transmission electron microscopy (TEM) analysis.
- Cai X, Lin M, Tan S, Mai W, Zhang Y, Liang Z, Lin Z, Zhang X (2012) The use of polyethyleneimine-modified reduced graphene oxide as a substrate for silver nanoparticles to produce a material with lower cytotoxicity and long-term antibacterial activity. Carbon 50(10):3407–3415. doi: 10.1016/j.carbon.2012.02.002 CrossRefGoogle Scholar
- Faria AF, Martinez DST, Moraes ACM, Maia da Costa MEH, Barros EB, Souza Filho AG, Paula AJ, Alves OL (2012) Unveiling the role of oxidation debris on the surface chemistry of graphene through the anchoring of Ag nanoparticles. Chem Mater 24(21):4080–4087. doi: 10.1021/cm301939s CrossRefGoogle Scholar
- Li J, Liu C (2010) Ag–graphene heterostructures: synthesis, characterization and optical properties. Eur J Inorg Chem:1099–0682. doi: 10.1002/ejic.200901048
- Yuan W, Jiang G, Che J, Qi X, Xu R, Chang MW, Chen Y, Lim SY, Dai J, Chan-Park MB (2008) Deposition of silver nanoparticles on multiwalled carbon nanotubes grafted with hyperbranched poly(amidoamine) and their antimicrobial effects. J Phys Chem C 112(48):18754–18759. doi: 10.1021/jp807133j CrossRefGoogle Scholar