Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells

  • L. A. Strobel
  • N. Hild
  • D. Mohn
  • W. J. Stark
  • A. Hoppe
  • U. Gbureck
  • R. E. Horch
  • U. Kneser
  • A. R. BoccacciniEmail author
Research Paper


The present study investigates a new family of bioactive glass nanoparticles with and without Sr-doping focusing on the influence of the nanoparticles on human bone marrow stromal cells (hBMSCs) in vitro. The bioactive glass nanoparticles were fabricated by flame spray synthesis and a particle diameter of 30–35 nm was achieved. Glass nanoparticles were undoped (BG 13-93-0Sr) or doped with 5 wt% strontium (Sr) (BG 13-93-5Sr) and used at concentrations of 10 and 100 μg/cm² (particles per culture plate area), respectively. Cells were cultured for 14 days after which the samples were analysed regarding metabolic activity and expression of various bone-specific genes. Cell growth and morphology indicated the high cytocompatibility of the nanoparticulate bioactive glass. The presence of the nanoparticles enhanced cell growth compared to the plain polystyrene control group. At a concentration of 100 μg/cm², Sr-doped particles led to significantly enhanced gene expression of osteocalcin, collagen type 1 and vascular endothelial growth factor. Thus, Sr-doped nanoparticles showing a dose-dependent increase of osteogenic differentiation in hBMSCs are a promising biomaterial for bone regeneration purposes.


Bioactive glass Human bone marrow stromal cells Nanoparticles Osteogenic differentiation Strontium Bone tissue engineering 



This work was partially supported by the Emerging Fields Initiative (EFI) of the University of Erlangen-Nuremberg, Germany (project “TOPbiomat”), and a research grant from the Deutsche Forschungsgemeinschaft DFG (KN 578/2-1). The authors gratefully thank Marina Milde for expert technical assistance.

Conflict of interest

None of the authors has any financial interest to declare in relation to the content of this article.

Supplementary material

11051_2013_1780_MOESM1_ESM.docx (103 kb)
Supplementary material 1 (DOCX 102 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • L. A. Strobel
    • 1
    • 2
  • N. Hild
    • 3
  • D. Mohn
    • 3
    • 4
  • W. J. Stark
    • 3
  • A. Hoppe
    • 5
  • U. Gbureck
    • 6
  • R. E. Horch
    • 1
  • U. Kneser
    • 1
    • 2
  • A. R. Boccaccini
    • 5
    Email author
  1. 1.Department of Plastic and Hand SurgeryUniversity of Erlangen-Nuremberg Medical CenterErlangenGermany
  2. 2.Department of Hand, Plastic and Reconstructive Surgery, Burn CentreBG Trauma CentreLudwigshafen/RhineGermany
  3. 3.Department of Chemistry and Applied Biosciences, Institute for Chemical and BioengineeringETH ZurichZurichSwitzerland
  4. 4.Department of Preventive Dentistry, Periodontology and Cariology, Center of Dental MedicineUniversity of ZurichZurichSwitzerland
  5. 5.Department of Materials Science and Engineering, Institute of BiomaterialsUniversity of Erlangen-NurembergErlangenGermany
  6. 6.Department for Functional Materials in Medicine and DentistryUniversity of WürzburgWürzburgGermany

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