Mesoporous silica nanoparticles as a biomolecule delivery vehicle in plants

  • Hashmath I. Hussain
  • Zhifeng Yi
  • James E. Rookes
  • Lingxue X. Kong
  • David M. Cahill
Research Paper

Abstract

We report the uptake by wheat, lupin and Arabidopsis of mesoporous silica nanoparticles functionalised with amine cross-linked fluorescein isothiocyanate (MSN-APTES-FITC). The preparation of these particles at room temperature enabled the synthesis of 20 nm particles that contained a network of interconnected pores around 2 nm in diameter. The uptake and distribution of these nanoparticles were examined during seed germination, in roots of plants grown in a hydroponic system and in whole leaves and roots of plants via vacuum infiltration. The nanoparticles did not affect seed germination in lupin and there was no phytotoxicity. Following germination of wheat and lupin grown in a nutrient solution containing nanoparticles, they were found within cells and cell walls of the emerging root and in the vascular transport elements, the xylem, and in other associated cells. In leaves and roots of Arabidopsis the nanoparticles were found, following vacuum infiltration of whole seedlings, to be taken up by the entire leaf and they were principally found in the intercellular spaces of the mesophyll but also throughout much of the root system. We propose that MSNs could be used as a novel delivery system for small molecules in plants.

Keywords

Mesoporous silica nanoparticles Fluorescein isothiocyanate 

Abbreviations

MSN

Mesoporous silica nanoparticle

MSN-APTES

MSN-aminopropyl triethoxysilane

MSN-APTES-FITC

MSN-APTES-fluorescein isothiocyanate

Supplementary material

11051_2013_1676_MOESM1_ESM.pptx (4.6 mb)
Fig. S1 Further examples of MSN-APTES-FITC by wheat roots. a bright field image of control root treated with MSN, b autofluorescene from the cell wall treated with MSN c bright field image of wheat root germinated in the presence of MSN-APTES-FITC, dwhite arrows indicates aggregates of MSN-APTES-FITC accumulated inside the cell and autofluorescence from the cell wall is observed, e bright field image of wheat root germinated in the presence of MSN-APTES-FITC where white arrows indicate the accumulation of aggregated MSN-FITC and f Accumulation of fluorescing MSN-APTES-FITC aggregates indicated by white arrowheads. Scale baraf (50 μm) (PPTX 4702 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Hashmath I. Hussain
    • 1
  • Zhifeng Yi
    • 2
  • James E. Rookes
    • 1
  • Lingxue X. Kong
    • 2
  • David M. Cahill
    • 1
  1. 1.Centre for Chemistry and Biotechnology, School of Life and Environmental SciencesDeakin UniversityGeelongAustralia
  2. 2.Institute for Frontier MaterialsDeakin UniversityGeelongAustralia

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