Advertisement

Interaction between serum albumins and sonochemically synthesized cadmium sulphide nanoparticles: a spectroscopic study

  • Selvaraj Naveenraj
  • Abdullah M. Asiri
  • Sambandam AnandanEmail author
Research Paper

Abstract

Cadmium Sulphide nanoparticles approximately 5–10 nm in size range were synthesized by sonochemical technique, which follows acoustic cavitation phenomenon and generates nanoparticles with a smaller size range and higher surface area. The in vitro binding interaction of these sonochemically synthesized CdS nanoparticles with serum albumins (SA) were investigated using UV–Vis absorption, fluorescence and circular dichroism (CD) spectroscopic techniques since CdS nanoparticles has biological applications such as cellular labelling and deep-tissue imaging. UV–Vis absorption and fluorescence studies confirm that CdS nanoparticles bind with SA through ground state complex formation (static quenching mechanism). The results suggest that sonochemically synthesized CdS nanoparticles interact with HSA more than that of BSA and these nanoparticles can be easily transported and rapidly released to the targets by serum albumins. CD studies confirmed the conformational change of serum albumins on the interaction of CdS nanoparticles.

Graphical Abstract

This paper investigates the in vitro binding interaction of Cadmium Sulphide (CdS) nanoparticles with serum albumins (HSA and BSA) using the UV-vis, steady-state fluorescence, time-resolved fluorescence, synchronous fluorescence and circular dichroism (CD) spectral techniques.

Keywords

Sonochemical synthesis CdS nanoparticles Serum albumins Fluorescence quenching Circular dichroism Interaction studies 

Notes

Acknowledgments

The author, SA thanks CSIR, New Delhi for the sanction major research Grant (CSIR reference No. 02(0021)/11/EMR-II) and the author, SA thank DST for sanctioning FIST (SR/FST/CSI-190/2008 dated 16th March 2009) and Nanomission (SR/NM/NS-27/2008, dated 25th Feb 2009) projects. The authors thank Prof. P. Ramamurthy, Director, the National Centre for Ultra Fast Processes, University of Madras, Chennai, India for time-resolved fluorescence measurements. Author SN thanks his institute for selecting him in MHRD fellowship.

References

  1. Arora S, Manoharan SS (2007) Size-dependent photoluminescent properties of uncapped CdS particles prepared by acoustic wave and microwave method. J. Phy. Chem. Solids 68:1897–1901CrossRefGoogle Scholar
  2. Ashokkumar M, Grieser F (2002) Sonochemical preparation of colloids. Encyclopedia of surface and colloid science. Taylor & Francis, Routledge, pp 4760–4774Google Scholar
  3. Atay Z, Biver T, Corti A, Eltugral N, Lorenzini E, Masini M, Paolicchi A, Pucci A, Ruggeri G, Secco F, Venturini M (2010) Non-covalent interactions of cadmium sulphide and gold nanoparticles with DNA. J Nanopart Res 12:2241–2253CrossRefGoogle Scholar
  4. Bang JH, Suslick KS (2010) Applications of ultrasound to the synthesis of nanostructured materials. Adv Mater 22:1039–1059CrossRefGoogle Scholar
  5. Benesi HA, Hildebrand JH (1949) A Spectrophotometric investigation of the interaction of Iodine with aromatic hydrocarbons. J Am Chem Soc 71:2703–2707CrossRefGoogle Scholar
  6. Dooley CJ, Rouge J, Ma N, Invernale M, Kelley SO (2007) Nucleotide-stabilized cadmium sulfide nanoparticles. J Mater Chem 17:1687–1691CrossRefGoogle Scholar
  7. Dufour C, Dangles O (2005) Flavonoid-serum albumin complexation: determination of binding constants and binding sites by fluorescence spectroscopy. Biochim Biophys Acta 1721:164–173CrossRefGoogle Scholar
  8. Gong W, Zheng Z, Zheng J, Gao W, Hu X, Ren X (2008) Luminescence study of water-soluble cds nanoparticles produced by femtosecond laser ablation at high laser fluence. J Phys Chem C 112:9983–9987CrossRefGoogle Scholar
  9. Jhonsi MA, Kathiravan A, Renganathan R (2009) Spectroscopic studies on the interaction of colloidal capped CdS nanoparticles with bovine serum albumin. Coll Surf B 72:167–172CrossRefGoogle Scholar
  10. Korgel BA, Monbouquette HG (1996) Synthesis of size-monodisperse CdS nanocrystals using phosphatidylcholine vesicles as true reaction compartments. J Phys Chem 100:346–351CrossRefGoogle Scholar
  11. Kumar A, Mandale AB, Sastry M (2000) Phase transfer of aqueous CdS nanoparticles by coordination with octadecanethiol molecules present in nonpolar organic solvents. Langmuir 16:9299–9302CrossRefGoogle Scholar
  12. Li J, Wu C, Gao F, Zhang R, Lv G, Fu D, Chenc B, Wang X (2006) In vitro study of drug accumulation in cancer cells via specific association with CdS nanoparticles. Bioorg Med Chem Lett 16:4808–4812CrossRefGoogle Scholar
  13. Li D, Zhu M, Xu C, Ji B (2011) Characterization of the baicalein-bovine serum albumin complex without or with Cu2+ or Fe3+ by spectroscopic approaches. Eur J Med Chem 46:588–599CrossRefGoogle Scholar
  14. Liu SH, Qian XF, Yin J, Ma XD, Yuan JY, Zhu ZK (2003) Preparation and characterization of polymer-capped CdS nanocrystals. J Phys Chem Solids 64:455–458CrossRefGoogle Scholar
  15. Lu W, Qin X, Luo Y, Chang G, Sun X (2011) CdS quantum dots as a fluorescent sensing platform for nucleic acid detection. Microchim Acta 175:355–359CrossRefGoogle Scholar
  16. Maleki M, Ghamsari MS, Mirdamadi S, Ghasemzadeh R (2007) A facile route for preparation of CdS nanoparticles. Semicond Phys Quantum Electron Optoelectron 10:30–32Google Scholar
  17. Moloto N, Revaprasadu N, Moloto MJ, O’Brien P, Helliwell M (2007) N, N′-Diisopropyl- and N, N′-dicyclohexylthiourea cadmium(II) complexes as precursors for the synthesis of CdS nanoparticles. Polyhedron 26:3947–3955CrossRefGoogle Scholar
  18. Naveenraj S, Anandan S, Kathiravan A, Renganathan R, Ashokkumar M (2010) The interaction of sonochemically synthesized gold nanoparticles with serum albumins. J Pharm Biomed Anal 53:804–810CrossRefGoogle Scholar
  19. Naveenraj S, Raj MR, Anandan S (2012) Binding Interaction between serum albumins and perylene-3,4,9,10-tetracarboxylate - a spectroscopic investigation. Dyes Pigment 94:330–337CrossRefGoogle Scholar
  20. Peretz S, Anghel DF, Teodor E, Stanciu G, Stoian C, Zgherea G, Florea- Spiroiu M (2011) Improving the properties of CdS nanoparticles by adding polymers. Part Sci Technol 29:229–241CrossRefGoogle Scholar
  21. Prabhu RR, Khadar MA (2005) Characterization of chemically synthesized CdS Nanoparticles. Pramana J Phys 65:801–807CrossRefGoogle Scholar
  22. Shao M, Xu F, Peng Y, Wu J, Li Q, Zhang S, Qian Y (2002) Microwave-templated synthesis of CdS nanotubes in aqueous solution at room temperature. New J Chem 26:1440–1442CrossRefGoogle Scholar
  23. Stern VO, Volmer M (1919) On the quenching-time of fluorescence. Physik Zeitsch 20:183–188Google Scholar
  24. Tang J, Qi S, Chen X (2005) Spectroscopic studies of the interaction of anti-coagulant rodenticide diphacinone with human serum albumin. J Mol Struct 779:87–95CrossRefGoogle Scholar
  25. Valeur B (2001) Molecular fluorescence: principles and applications. Wiley, New York, pp 247–272CrossRefGoogle Scholar
  26. Wang GZ, Chen W, Liang CH, Wang YW, Meng GW, Zhang LD (2001) Preparation and characterization of CdS nanoparticles by ultrasonic irradiation. Inorg Chem Commun 4:208–210CrossRefGoogle Scholar
  27. Wang G, Wang D, Li X, Lu Y (2011) Exploring the binding mechanism of dihydropyrimidinones to human serum albumin: Spectroscopic and molecular modeling techniques. Colloids Surf B 84:272–279CrossRefGoogle Scholar
  28. Weiss S (1999) Fluorescence spectroscopy of single biomolecules. Science 283:1676–1683CrossRefGoogle Scholar
  29. Wu Y, Wang L, Xiao M, Huang X (2008) A novel sonochemical synthesis and nanostructured assembly of polyvinylpyrrolidone-capped CdS colloidal nanoparticles. J Non-Cryst Solids 354:2993–3000CrossRefGoogle Scholar
  30. Yue Y, Chen X, Qin J, Yao X (2009) Spectroscopic investigation on the binding of antineoplastic drug oxaliplatin to human serum albumin and molecular modeling. Colloids Surf B 69:51–57CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Selvaraj Naveenraj
    • 1
  • Abdullah M. Asiri
    • 2
  • Sambandam Anandan
    • 1
    Email author
  1. 1.Nanomaterials & Solar Energy Conversion Lab, Department of ChemistryNational Institute of TechnologyTiruchirappalliIndia
  2. 2.The Center of Excellence for Advanced Materials Research (CEAMR)King Abdulaziz UniversityJiddaSaudi Arabia

Personalised recommendations