A Promising Combo Gene Delivery System Developed from (3-Aminopropyl)triethoxysilane-Modified Iron Oxide Nanoparticles and Cationic Polymers
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(3-Aminopropyl)triethoxysilane-modified iron oxide nanoparticles (APTES-IONPs) have been evaluated for various biomedical applications, including medical imaging and drug delivery. Cationic polymers (CPs) such as Lipofectamine and TurboFect are widely used for research in gene delivery, but their toxicity and low in vivo efficiency limited their further application. In the present study, we synthesized water-soluble APTES-IONPs and developed a combo gene delivery system based on APTES-IONPs and CPs. This system significantly increased gene-binding capacity, protected genes from degradation, and improved gene transfection efficiency for DNA and siRNA in both adherent and suspension cells. Because of its great biocompatibility, high gene-carrying ability, and very low cytotoxicity, this combo gene delivery system will be expected for a wide application, and it might provide a new method for gene therapy.
KeywordsIron oxide nanoparticles (3-Aminopropyl)trimethoxysilane (APTES) Cationic polymers Leukemia Gene delivery
This project was supported by the National Basic Research Program of China (Program “973”, 2011CB933501), by the National Natural Science Foundation of China (Grants No. 81071935, 81272632, 81101795), by the Natural Science Foundation of Jiangsu Province (Grants No. BK2010218, BK2011268), and by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
- Cheang TY, Tang B, Xu AW, Chang GQ, Hu ZJ, He WL, Xing ZH, Xu JB, Wang M, Wang SM (2011) Promising plasmid DNA vector based on APTES-modified silica nanoparticles. Int J Nanomedicine 7:1061–1067Google Scholar
- Ling D, Hyeon T (2012) Chemical design of biocompatible iron oxide nanoparticles for medical applications. Small. doi: 10.1002/smll.201202111
- Mao X, Cao B, Wood TE, Hurren R, Tong J, Wang X, Wang W, Li J, Jin Y, Sun W, Spagnuolo PA, MacLean N, Moran MF, Datti A, Wrana J, Batey RA, Schimmer AD (2011) A small-molecule inhibitor of d-cyclin transactivation displays preclinical efficacy in myeloma and leukemia via phosphoinositide 3-kinase pathway. Blood 117(6):1986–1997CrossRefGoogle Scholar
- Meng Lin M, Kim HH, Kim H, Muhammed M, Kyung Kim D (2010) Iron oxide-based nanomagnets in nanomedicine: fabrication and applications. Nano Rev 1. doi: 10.3402/nano.v1i0.4883
- Singh KK (2005) Nanotechnology in cancer detection and treatment. Technol Cancer Res Treat 4(6):583Google Scholar
- Wang S, Wen S, Shen M, Guo R, Cao X, Wang J, Shi X (2011) Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay. Int J Nanomedicine 6:3449–3459Google Scholar