Capture of unstable protein complex on the streptavidin-coated single-walled carbon nanotubes
Purification of unstable protein complexes is a bottleneck for investigation of their 3D structure and in protein–protein interaction studies. In this paper, we demonstrate that streptavidin-coated single-walled carbon nanotubes (Strep•SWNT) can be used to capture the biotinylated DNA–EcoRI complexes on a 2D surface and in solution using atomic force microscopy and electrophoresis analysis, respectively. The restriction enzyme EcoRI forms unstable complexes with DNA in the absence of Mg2+. Capturing the EcoRI–DNA complexes on the Strep•SWNT succeeded in the absence of Mg2+, demonstrating that the Strep•SWNT can be used for purifying unstable protein complexes.
KeywordsUnstable protein complex Carbon nanotube Streptavidin EcoRI
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