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Comparison of TiO2 and ZnO nanoparticles for photocatalytic degradation of methylene blue and the correlated inactivation of gram-positive and gram-negative bacteria

  • Robert J. Barnes
  • Rodrigo Molina
  • Jianbin Xu
  • Peter J. Dobson
  • Ian P. ThompsonEmail author
Research Paper

Abstract

Titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles are important photocatalysts and as such have been extensively studied for the removal of organic compounds from contaminated air and water and for microbial disinfection. Despite much research on the effect of TiO2 and ZnO nanoparticles on different bacterial species, uncertainties remain about which bacteria are more sensitive to these compounds. Very few studies have directly compared the toxicity of ZnO to TiO2 under both light and dark conditions. In addition, authors investigating the photocatalytic inactivation of TiO2 and ZnO nanoparticles on bacteria have failed to investigate the reactive oxygen species (ROS) generation of the nanoparticles, making it difficult to correlate killing action with the generation of ROS. In this study, three types of metal nanoparticle (ZnO < 50 nm, ZnO < 100 nm and TiO2) have been characterised and ROS production assessed through the degradation of methylene blue (MB). The photocatalytic killing potential of three nanoparticle concentrations (0.01, 0.1 and 1 g/L) was then assessed on four representative bacteria: two gram-positive (S. aureus and B. subtilis) and two gram-negative (E. coli and P. aeruginosa). Results showed that out of the three nanoparticles tested, the TiO2 nanoparticles generated more ROS than the ZnO nanoparticles, corresponding to a greater photocatalytic inactivation of three of the four species of bacteria examined. The MB decomposition results correlated well with the bacterial inactivation results with higher TiO2 nanoparticle concentrations leading to greater ROS production and increased loss of cell viability. Although producing less ROS than the TiO2 nanoparticles under ultraviolet light, the ZnO nanoparticles were toxic to two of the bacterial species even under dark conditions. In this study, no correlation between cell wall type and bacterial inactivation was observed for any of the nanoparticles tested although both gram-positive bacteria were sensitive to ROS production. P. aeruginosa cells were resistant to all types of treatment and highlight a potential limitation to the application of these nanoparticles for water treatment.

Keywords

Nanoparticles Metal oxides Reactive oxygen species Toxicity Bacteria Photocatalysis 

Notes

Acknowledgments

Research was supported by NERC. The authors also thank Xin Zhao and Frank Cullen of the Department of Materials, University of Oxford, for TEM and XRD analyses.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Robert J. Barnes
    • 1
  • Rodrigo Molina
    • 1
  • Jianbin Xu
    • 1
  • Peter J. Dobson
    • 2
  • Ian P. Thompson
    • 1
    Email author
  1. 1.Department of Engineering ScienceOxford UniversityOxfordUK
  2. 2.Begbroke Directorate, Oxford University Begbroke Science ParkSandy LaneUK

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