Journal of Nanoparticle Research

, 14:1265

In vitro and in vivo investigations on bone regeneration potential of laminated hydroxyapatite/gelatin nanocomposite scaffold along with DBM

  • Shima Tavakol
  • Iraj Ragerdi Kashani
  • Mahmood Azami
  • Ahad Khoshzaban
  • Behnaz Tavakol
  • Sharmin Kharrazi
  • Somayeh Ebrahimi
  • Seyed Mahdi Rezayat Sorkhabadi
Research Paper


Bone regeneration ability of a scaffold strongly depends on its structure and the size of its components. In this study, a nanostructured scaffold was designed for bone repair using nano hydroxyapatite (nHA) (8–16 nm × 50–80 nm) and gelatin (GEL) as main components. In vitro investigations of calcium matrix deposition and gene expression of the seeded cells for this scaffold, demineralized bone matrix (DBM), scaffold plus DBM, and the control group were carried out. Bone regeneration in rat calvarium with critical defect size after 1, 4, and 8 weeks post implantation was investigated. The calcium matrix depositions by the osteoblast and RUNX2, ALP, osteonectin, and osteocalcin gene expression in scaffold were more significant than in other groups. Histomorphometry analysis confirmed in vitro results. In vitro and in vivo bone regeneration were least in scaffold plus DBM group. Enhanced effects in scaffold could be attributed to the shape and size of nHA particles and good architecture of the scaffold. Reduction of bone regeneration might be due to tight bonding of BMPs and nHA particles in the third group. Results obtained from this study confirmed that nano-scale size of the main components and the scaffold architecture (pore diameter, interconnectivity pores, etc.) have significant effects on bone regeneration ability of the scaffold and are important parameters in designing a temporary bone substitute.


Scaffold Nano hydroxyapatite Demineralized bone matrix Bone regeneration Gene expression Histomorphometry 


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Shima Tavakol
    • 1
  • Iraj Ragerdi Kashani
    • 2
  • Mahmood Azami
    • 3
  • Ahad Khoshzaban
    • 4
  • Behnaz Tavakol
    • 5
  • Sharmin Kharrazi
    • 1
  • Somayeh Ebrahimi
    • 6
  • Seyed Mahdi Rezayat Sorkhabadi
    • 1
    • 7
    • 8
  1. 1.Department of Medical NanotechnologySchool of Advanced Technologies in Medicine, Tehran University of Medical SciencesTehranIran
  2. 2.Department of AnatomySchool of Medicine, Tehran University of Medical SciencesTehranIran
  3. 3.Department of Tissue EngineeringSchool of Advanced Technologies in Medicine, Tehran University of Medical SciencesTehranIran
  4. 4.Iranian Tissue Bank Research & Preparation CenterTehran University of Medical SciencesTehranIran
  5. 5.Department of MedicineKashan University of Medical SciencesKashanIran
  6. 6.Department of Biology, Faculty of SciencesUniversity of Tarbiat MoallemTehranIran
  7. 7.Department of Toxicology & Pharmacology, Faculty of PharmacyPharmaceutical Sciences Branch, Islamic Azad University (IAUPS)TehranIran
  8. 8.Department of Pharmacology, Faculty of MedicineTehran University of Medical SciencesTehranIran

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