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Cellular uptake and cytotoxicity of positively charged chitosan gold nanoparticles in human lung adenocarcinoma cells

  • Seon Young Choi
  • Soo Hwa Jang
  • Jin Park
  • Saeromi Jeong
  • Jin Ho Park
  • Kwang Su Ock
  • Kangtaek Lee
  • Sung Ik Yang
  • Sang-Woo JooEmail author
  • Pan Dong Ryu
  • So Yeong LeeEmail author
Research Paper

Abstract

Cellular uptake, cytotoxicity, and mechanisms of cytotoxicity of the positively charged Au nanoparticles (NPs) were examined in A549 cells, which are one of the most characterized pulmonary cellular systems. Positively charged Au NPs were prepared by chemical reduction using chitosan. The dimension and surface charge of Au NPs were examined by transmission electron microscopy (TEM), dynamic light scattering, and zeta potential measurements. The uptake of Au NPs into A549 cells was also monitored using TEM and dark-field microscopy (DFM) and z-stack confocal microRaman spectroscopy. DFM live cell imaging was also performed to monitor the entry of chitosan Au NPs in real time. The cytotoxic assay, using both methylthiazol tetrazolium and lactate dehydrogenase assays revealed that positively charged Au NPs decreased cell viability. Flow cytometry, DNA fragmentation, real-time PCR, and western blot analysis suggest that positively charged chitosan Au NPs provoke cell damage through both apoptotic and necrotic pathways.

Keywords

Nanoparticles Au Chitosan Cellular uptake Cytotoxicity 

Notes

Acknowledgments

We acknowledge the financial support from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2012-0000906, 2011-0027696, 2011-0014514) and the Development of Characterization Techniques for Nano-materials Safety Project of KRCF. This subject is supported by Korea Ministry of Environment as “Environmental Health R&D Program”.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Seon Young Choi
    • 1
  • Soo Hwa Jang
    • 1
  • Jin Park
    • 2
  • Saeromi Jeong
    • 2
  • Jin Ho Park
    • 2
  • Kwang Su Ock
    • 2
  • Kangtaek Lee
    • 3
  • Sung Ik Yang
    • 4
  • Sang-Woo Joo
    • 2
    Email author
  • Pan Dong Ryu
    • 1
  • So Yeong Lee
    • 1
    Email author
  1. 1.Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Institute for Veterinary ScienceSeoul National UniversitySeoulSouth Korea
  2. 2.Department of ChemistrySoongsil UniversitySeoulSouth Korea
  3. 3.Department of Chemical and Biomolecular EngineeringYonsei UniversitySeoulSouth Korea
  4. 4.College of Environment and Applied ChemistryKyung Hee UniversityYonginSouth Korea

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