Development of a gold-nanostructured surface for amperometric genosensors
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A gold-nanostructured surface has been obtained by stable deposition of chemically synthesized gold nanoparticles (2.1–5.5 nm size range) on a gold substrate through a dithiol linker. The method proposed for the obtainment of the nanostructure is suitable for the further stable anchoring of a peptide nucleic acid oligomer through four amine groups of lysine terminal residues, leading to fairly reproducible systems. The geometric area of the nanostructured surface is compared with those of a smooth and of an electrochemically generated nanostructured surface by depositing a probe bearing an electrochemically active ferrocene residue. Despite the area of the two nanostructures being quite similar, the response toward a 2 nM target oligonucleotide sequence is particularly high when using the surface built up by nanoparticle deposition. This aspect indicates that morphologic details of the nanostructure play a key role in conditioning the performances of the genosensors.
KeywordsGold nanoparticles Nanostructured surface Amperometric genosensor Peptide nucleic acid Ferrocene derivatives Amine deposition
Dr. Massimo Tonelli of the Centro Interdipartimentale Grandi Strumenti (CIGS—Università di Modena e Reggio Emilia) is acknowledged for the acquisition of the SEM images. The CIGS is also acknowledged for the use of the TEM instrument. C.Z. and R.S. acknowledge the Ministero dell’Istruzione, dell’Università e della Ricerca (MIUR, Rome) for the financial support from PRIN 2009 (2009YRH27R). E.L. wishes to thank the MIUR (Rome) and the University of Milan for the financial support from PRIN 2007 (2007F9TWKE_002) and PRIN 2009 (20093N774P_003). C.B. wishes to thank CNR-PM.P06.003.
- Carralero Sanz V, Luz Mena M, González-Cortés A, Yánez-Sedeno P, Pingarrón JM (2005) Development of a tyrosinase biosensor based on gold nanoparticles-modified glassy carbon electrodes. Application to the measurement of a bioelectrochemical polyphenols index in wines. Anal Chim Acta 528:1–8CrossRefGoogle Scholar
- Cederquist KB, Keating CD (2009) Curvature effects in DNA: Au nanoparticle conjugates. ACSNano 3:256–260Google Scholar
- Finklea HO (2003) Electrochemistry of organized monolayers of thoils and related molecules on electrodes. In: Bard A, Robinstein I (eds) Electroanalytical chemistry, vol 19. Marcel Dekker, New York, pp 109–335Google Scholar
- Hejazi MS, Pournaghi-Azar MH, Alipour E, Abdolahinia ED, Arami S, Navvah H (2011) Development of a novel electrochemical biosensor for detection and discrimination of DNA sequence and single base mutation in dsDNA samples based on PNA-dsDNA hybridization—a new platform technology. Electroanalysis 23:503–511CrossRefGoogle Scholar
- Hill HD, Millstone JE, Banholzer MJ, Mirkin CA (2009) The role radius of curvature plays in thiolated oligonucleotide loading on gold nanoparticles. ACSNano 3:418–424Google Scholar
- Mateo-Martí E, Pradier C (2010) A novel type of nucleic acid-based biosensors: the use of PNA probes, associated with surface science and electrochemical detection techniques. In: Somerset VS (ed) Intelligent and biosensors. InTech, Open Access Publisher, USA, pp 323–344Google Scholar
- Moretto L, Panero S, Scrosati B, Ugo P (2009) Template ensemble of nanoelectrodes. In: Lin Y, Nalwa HS (eds) Handbook of electrochemical technology, vol 1. American Scientific Publisher, California, pp 87–105Google Scholar
- Nielsen PE (2004) Peptide nucleic acids: protocols and applications, 2nd edn. Horizon Bioscience, WymondhamGoogle Scholar
- Pasquali L, Terzi F, Zanardi C, Seeber R, Paolicelli G, Mahne N, Nannarone S (2007b) Bonding and orientation of 1,4-benzenedimethanethiol on Au(111) prepared from solution and from gas phase. J Physics C 19:305020Google Scholar
- Pournaghi-Azar MH, Ahour F, Hejazi MS (2010) Direct detection and discrimination of double-stranded oligonucleotide corresponding to hepatitis C virus genotype 3a using an electrochemical DNA biosensor based on peptide nucleic acid and double-stranded DNA hybridization. Anal Bioanal Chem 397:3581–3587CrossRefGoogle Scholar
- Zanardi C, Terzi F, Seeber R, Baldoli C, Licandro E, Maiorana S (2012) Peptide nucleic acid tagged with four lysine residues for amperometric genosensors. Artificial DNA 3:80–87Google Scholar