Manganese–gold nanoparticles as an MRI positive contrast agent in mesenchymal stem cell labeling

  • Simona E. Hunyadi Murph
  • Stephanie Jacobs
  • Jimei Liu
  • Tom C.-C. Hu
  • Matthew Siegfired
  • Steven M. Serkiz
  • Joan Hudson
Research Paper

DOI: 10.1007/s11051-011-0658-7

Cite this article as:
Murph, S.E.H., Jacobs, S., Liu, J. et al. J Nanopart Res (2012) 14: 658. doi:10.1007/s11051-011-0658-7

Abstract

We report a straightforward approach to prepare multifunctional manganese–gold nanoparticles by attaching Mn(II) ions onto the surface of 20 nm citrate-capped gold nanoparticles. In vitro MRI measurements made in agarose gel phantoms exhibited high relaxivity (18.26 ± 1.04 mmol−1 s−1). Controlled incubation of the nanoparticles with mesenchymal stem cells (MSCs) was used to study cellular uptake of these particles and this process appeared to be controlled by the size of the nanoparticle aggregates in the extracellular solution. SEM images of live MSCs showed an increased concentration of particles near the cell membrane and a distribution of the size of particles within the cells. Survivability for MSCs in contact with Mn–Au NPs was greater than 97% over the 3-day period and up to the 1 mM Mn used in this study. The high relaxivity and low cell mortality are suggestive of an enhanced positive contrast agent for in vitro or in vivo applications.

Keywords

Manganese Gold Imaging MRI Multifunctional nanoparticles Stem cell uptake Synthesis Nanomedicine 

Supplementary material

11051_2011_658_MOESM1_ESM.doc (1.3 mb)
Supplementary material 1 (DOC 1350 kb)

Copyright information

© Springer Science+Business Media B.V. (outside the USA) 2012

Authors and Affiliations

  • Simona E. Hunyadi Murph
    • 1
    • 4
  • Stephanie Jacobs
    • 2
  • Jimei Liu
    • 2
  • Tom C.-C. Hu
    • 2
  • Matthew Siegfired
    • 1
  • Steven M. Serkiz
    • 1
    • 3
  • Joan Hudson
    • 3
  1. 1.Savannah River National LaboratoryAikenUSA
  2. 2.Department of Radiology, Small Animal ImagingMedical College of GeorgiaAugustaUSA
  3. 3.School of Materials Science and EngineeringClemson UniversityClemsonUSA
  4. 4.Savannah River National LaboratoryAikenUSA

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