Journal of Nanoparticle Research

, Volume 13, Issue 12, pp 6673–6681 | Cite as

Encapsulation of iron nanoparticles in alginate biopolymer for trichloroethylene remediation

  • Achintya N. Bezbaruah
  • Sai Sharanya Shanbhogue
  • Senay Simsek
  • Eakalak Khan
Research Paper


Nanoscale zero-valent iron (NZVI) particles (10–90 nm) were encapsulated in biodegradable calcium-alginate capsules for the first time for application in environmental remediation. Encapsulation is expected to offers distinct advances over entrapment. Trichloroethylene (TCE) degradation was 89–91% in 2 h, and the reaction followed pseudo first order kinetics for encapsulated NZVI systems with an observed reaction rate constant (k obs) of 1.92–3.23 × 10−2 min−1 and a surface normalized reaction rate constant (k sa) of 1.02–1.72 × 10−3 L m−2 min−1. TCE degradation reaction rates for encapsulated and bare NZVI were similar indicating no adverse affects of encapsulation on degradation kinetics. The shelf-life of encapsulated NZVI was found to be four months with little decrease in TCE removal efficiency.


Nanoscale zero-valent iron (NZVI) Encapsulated NZVI Trichloroethylene (TCE) Calcium-alginate Biopolymer Environmental remediation 



This research was supported by USGS/North Dakota Water Resources Research Institute (NDWRRI). The help from the members (especially Mr. Harjyoti Kalita and Ms. Sita Krajangpan) of Nanoenvirology Research Group (NRG) and Environmental Engineering Laboratory of North Dakota State University is thankfully acknowledged.


  1. Aksu Z, E-retli G, Kutsal T (2002) A comparative study of copper(II) biosorption on Ca-alginate, agarose and immobilized C. vulgaris in a packed-bed column. Process Biochem 33:393–400CrossRefGoogle Scholar
  2. Alowitz MJ, Scherer MM (2002) Kinetics of nitrate, nitrite, and Cr(VI) reduction by iron metal. Environ Sci Technol 36:299–306CrossRefGoogle Scholar
  3. APHA, AWWA, WEF (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, WashingtonGoogle Scholar
  4. Augst AD, Kong HJ, Mooney D (2006) Alginate hydrogels as biomaterials. Macromol Biosci 6:623–633CrossRefGoogle Scholar
  5. Bayramoğlu G, Arica Y (2009) Construction a hybrid biosorbent using Scenedesmus quadricauda and Ca-alginate for biosorption of Cu(II), Zn(II) and Ni(II): kinetics and equilibrium studies. Bioresour Technol 100:186–193CrossRefGoogle Scholar
  6. Bezbaruah AN, Krajangpan S, Chisholm BJ, Khan E, Bermudez JJ (2009a) Entrapment of iron nanoparticles in calcium alginate beads for groundwater remediation applications. J Hazard Mater 166:1339–1343CrossRefGoogle Scholar
  7. Bezbaruah AN, Thompson JM, Chisholm BJ (2009b) Remediation of alachlor and atrazine contaminated water with zero-valent iron nanoparticles. J Environ Sci Health Part B Pestic Food Contam Agric Wastes 44:518–524Google Scholar
  8. Bleve G, Lezzi C, Chiriatti MA, D’Ostuni I, Tristezza M, Di Venere D, Sergio L, Mita G, Grieco F (2011) Selection of non-conventional yeasts and their use in immobilized form for the bioremediation of olive oil mill wastewater. Bioresour Technol 102:982–989CrossRefGoogle Scholar
  9. Chan ES, Yim ZH, Phan SH, Mansa RF, Ravindra P (2010) Encapsulation of herbal aqueous extract through absorption with Ca-alginate hydrogel beads. Food Bioprod Process 88:195–201CrossRefGoogle Scholar
  10. Garbayo I, Leon R, Vigara J, Vılchez C (2002) Diffusion characteristics of nitrate and glycerol in alginate. Colloid Surf B 25:1–9CrossRefGoogle Scholar
  11. Gregory KB, Larese-Casanova P, Parkin GF, Scherer MM (2004) Abiotic transformation of hexahydro-1,3,5-trinitro-1,3,5-triazine by FeII bound to magnetite. Environ Sci Technol 38:1408–1414CrossRefGoogle Scholar
  12. He F, Zhao DY, Paul C (2010) Field assessment of carboxymethyl cellulose stabilized iron nanoparticles for in situ destruction of chlorinated solvents in source zones. Water Res 44:2360–2370CrossRefGoogle Scholar
  13. Hill CB, Khan E (2008) A comparative study of immobilized nitrifying and co immobilized nitrifying and denitrifying bacteria for ammonia removal from sludge digester supernatant. Water Air Soil Pollut 195:23–33CrossRefGoogle Scholar
  14. Huang GL, Zhihui S (2002) Immobilization of Spirulina subsalsa for removal of triphenyltin from water. Artif Cells Blood Substit Immobil Biotechnol 30:293–305CrossRefGoogle Scholar
  15. Johnson TL, Scherer MM, Tratnyek PG (1996) Kinetics of halogenated organic compound degradation by iron metal. Environ Sci Technol 30:2634–2640CrossRefGoogle Scholar
  16. Joo SH, Zhao D (2008) Destruction of lindane and atrazine using stabilized iron nanoparticles under aerobic and anaerobic conditions: effects of catalyst and stabilizer. Chemosphere 70:418–425CrossRefGoogle Scholar
  17. Kim H, Hong HJ, Jung J, Kim SH, Yang JW (2010) Degradation of trichloroethylene (TCE) by nanoscale zero-valent iron (nZVI) immobilized in alginate bead. J Hazard Mater 176:1038–1043CrossRefGoogle Scholar
  18. Lai YL, Annadurai G, Huang FC, Lee JF (2008) Biosorption of Zn(II) on the different Ca-alginate beads from aqueous solution. Bioresour Technol 99:6480–6487CrossRefGoogle Scholar
  19. Lin YB, Fugetsu B, Terui N, Tanaka S (2005) Removal of organic compounds by alginate gel beads with entrapped activated carbon. J Hazard Mater 120:237–241CrossRefGoogle Scholar
  20. Liu YQ, Lowry GV (2006) Effect of particle age (Fe0 content) and solution pH on NZVI reactivity: H2 evolution and TCE dechlorination. Environ Sci Technol 40:6085–6090CrossRefGoogle Scholar
  21. Liu Y, Majetich SA, Tilton RD, Sholl DS, Lowry GV (2005) TCE dechlorination rates, pathways, and efficiency of nanoscale iron particles with different properties. Environ Sci Technol 39:1338–1345CrossRefGoogle Scholar
  22. Lu Y, Xu S, Jiang Z, Yuan W, Wang T (2005) Diffusion of nicotinamide adenine dinucleotide in calcium alginate hydrogel beads doped with carbon and silica nanotubes. J Chem Eng Data 50:1319–1323CrossRefGoogle Scholar
  23. Matheson LJ, Tratnyek PG (1994) Reductive dehalogenation of chlorinated methanes by iron metal. Environ Sci Technol 28:2045–2053CrossRefGoogle Scholar
  24. Önal S, Baysal SH, Ozdemir G (2007) Studies on the applicability of alginate entrapped Chryseomonas luteola TEM 05 for heavy metal biosorption. J Hazard Mater 146:417–420CrossRefGoogle Scholar
  25. Phenrat T, Liu Y, Tilton RD, Lowry GV (2009a) Adsorbed polyelectrolyte coatings decrease Fe0 nanoparticle reactivity with TCE in water conceptual model and mechanisms. Environ Sci Technol 43:1507–1514CrossRefGoogle Scholar
  26. Phenrat T, Long TC, Lowry GV, Veronsei B (2009b) Partial oxidation (“aging”) and surface modification decrease the toxicity of nanosized zerovalent iron. Environ Sci Technol 43:195–200CrossRefGoogle Scholar
  27. Pramanik S, McEvoy J, Siripattanakul S, Khan E (2011) Effects of entrapment on nucleic acid content and microbial diversity of mixed cultures in biological wastewater treatment. Bioresour Technol 102:3176–3183CrossRefGoogle Scholar
  28. Schrick B, Blough JL, Jones AD, Mallouk TE (2002) Hydrodechlorination of trichloroethylene to hydrocarbons using bimetallic nickel-iron nanoparticles. Chem Mater 14:5140–5147CrossRefGoogle Scholar
  29. Srimornsak P, Sungthongjeen S (2007) Modification of theophylline release with alginate gel formed in hard capsules. Pharm Sci Tech 8:1–8CrossRefGoogle Scholar
  30. Tanriseven A, Doan S (2001) Immobilization of invertase within calcium alginate gel capsules. Process Biochem 36:1081–1083CrossRefGoogle Scholar
  31. Thompson JM, Chisholm BJ, Bezbaruah AN (2010) Reductive dechlorination of chloroacetanilide herbicide (alachlor) using zero-valent iron nanoparticles. Environ Eng Sci 27:227–232CrossRefGoogle Scholar
  32. USEPA (1992) Measurement of purgable organic compounds in water by capillary column gas chromatography/mass spectrometry, Method 524.2. Environmental Monitoring Systems Laboratory, Office of Research and Development, United Stated Environmental Protection Agency, OhioGoogle Scholar
  33. Wang CB, Zhang WX (1997) Synthesizing nanoscale iron particles for rapid and complete dechlorination of TCE and PCBs. Environ Sci Technol 31:2154–2156CrossRefGoogle Scholar
  34. Wang W, Zhou M, Jin Z, Li T (2010) Reactivity characteristics of poly (methyl methacrylate) coated nanoscale iron particles for trichloroethylene remediation. J Hazard Mater 173:724–730CrossRefGoogle Scholar
  35. Wang J, Jin Y, Liu J, Ju X, Meng T, Chu L (2011) Novel calcium-alginate capsules with aqueous core and thermo-responsive membrane. J Colloid Interface Sci 353:61–68CrossRefGoogle Scholar
  36. Westrin BA, Axelsson A (1991) Diffusion in gels containing immobilized cells: a critical review. Biotechnol Bioeng 38:439–446CrossRefGoogle Scholar
  37. Zhang WX (2003) Nanoscale iron particles for environmental remediation: an overview. J Nanopart Res 5:323–332CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Achintya N. Bezbaruah
    • 1
    • 2
  • Sai Sharanya Shanbhogue
    • 1
    • 2
  • Senay Simsek
    • 3
  • Eakalak Khan
    • 2
  1. 1.Nanoenvirology Research Group (NRG)North Dakota State UniversityFargoUSA
  2. 2.Department of Civil EngineeringNorth Dakota State UniversityFargoUSA
  3. 3.Department of Plant SciencesNorth Dakota State UniversityFargoUSA

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