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Journal of Nanoparticle Research

, Volume 13, Issue 9, pp 4075–4083 | Cite as

Phosphorylcholine functionalized dendrimers for the formation of highly stable and reactive gold nanoparticles and their glucose conjugation for biosensing

  • Lan Jia
  • Li-Ping Lv
  • Jian-Ping Xu
  • Jian Ji
Research Paper

Abstract

Phosphorylcholine (PC)-functionalized poly(amido amine) (PAMAM) dendrimers were prepared and used as both reducing and stabilizing agents for synthesis of highly stable and reactive gold nanoparticles (Au NPs). Biomimetic PC-functionalized PAMAM dendrimers-stabilized gold nanoparticles (Au DSNPs) were formed by simply mixing the PC modified amine-terminated fifth-generation PAMAM dendrimers (G5-PC) with AuCl4 ions by controlling the pH, no additional reducing agents or other stabilizers were needed. The obtained Au DSNPs were shown to be spherical, with particle diameters ranging from 5 to 12 nm, the sizes and growth kinetics of Au DSNPs could be tuned by changing the pH and the initial molar ratio of dendrimers to gold as indicated by transmission electron microscopy (TEM) and UV–Vis data. The prepared Au DSNPs showed excellent stability including: (1) stable at wide pH (7–13) values; (2) stable at high salt concentrations up to 2 M NaCl; (3) non-specific protein adsorption resistance. More importantly, surface functionalization could be performed by introducing desired functional groups onto the remained reactive amine groups. This was exemplified by the glucose conjugation. The glucose conjugated Au DSNPs showed bio-specific interaction with Concanavalin A (Con A), which induced aggregation of the Au NPs. Colorimetric detection of Con A based on the plasmon resonance of the glucose conjugated Au DSNPs was realized. A limit of detection (LOD) for Con A was 0.6 μM, based on a signal-to-noise ratio (S/N) of 3. These findings demonstrated that the PC modified Au DSNPs could potentially serve as a versatile nano-platform for the biomedical applications.

Keywords

Biomimetic Poly (amido amine) Biosensors Stability Colorimetric Gold nanoparticles Nanomedicine 

Notes

Acknowledgments

This research was financially supported by Natural Science Foundation of China (NSFC-20774082, 50703036, 50830106), National Science Fund for Distinguished Young Scholars (51025312), Zhejiang Provincial Natural Science Foundation of China (Y4080024, Y4080250), Ph.D. Programs Foundation of Ministry of Education (No. 20070335024) and Qianjiang Excellence Project of Zhejiang Province (2009R10051).

Supplementary material

11051_2011_351_MOESM1_ESM.doc (1.6 mb)
Supplementary material 1 (DOC 1685 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of Polymer Science and Engineering, MOE Key Laboratory of Macromolecular Synthesis and FunctionalizationZhejiang UniversityHangzhouChina

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