Studies on toxicity of aluminum oxide (Al2O3) nanoparticles to microalgae species: Scenedesmus sp. and Chlorella sp.
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In view of increasing commercial applications of metal oxide nanoparticles their toxicity assessment becomes important. Alumina (Al2O3) nanoparticles have wide range of applications in industrial as well as personal care products. In the absence of prior report on toxicological impact of alumina nanoparticles to microalgae, the principal objective of this study was to demonstrate the effect of the nanoparticles on microalgae isolated from aquatic environment (Scenedesmus sp. and Chlorella sp.). The growth inhibitory effect of alumina nanoparticles was observed for both the species (72 h EC50 value, 45.4 mg/L for Chlorella sp.; 39.35 mg/L for Scenedesmus sp.). Bulk alumina also showed toxicity though to a lesser extent (72 h EC50 value, 110.2 mg/L for Chlorella sp.; 100.4 mg/L for Scenedesmus sp.). A clear decrease in chlorophyll content was observed in the treated cells compared to the untreated ones, more effect being notable in the case of nanoparticles. Preliminary results based on FT-IR studies, optical and scanning electron microscopic images suggest interaction of the nanoparticles with the cell surface.
KeywordsNanoparticles Alumina Microalgae EC50 Shading effect Aggregates Surface interactions Health effects EHS
- Chen KL, Elimelech M (2007) Influence of humic acid on the aggregation kinetics of fullerene (C60) nanoparticles in monovalent and divalent electrolyte solutions. J Colloid Interface Sci 309(1):126–134Google Scholar
- Filella M, Buffle J (1993) Factors controlling the stability of submicron colloids in natural waters. Colloids Surf A: Physicochem Eng Aspects 73:255–273Google Scholar
- Hoeckel V, DeSchamphelaere K, Vander Meeren KAC, Lucas P, Janssen SCR (2008) The ecotoxicity of silica nanoparticles to the alga Pseudokirchneriella subcapitata: importance of surface area. Environ Toxicol Chem 27:127–136Google Scholar
- Huang CP, Cha DK, Ismat SS (2005) Progress report: short-term chronic toxicity of photocatalytic nanoparticles to bacteria, algae, and zooplankton. EPA Grant Number: R831721. http://cfpub.epa.gov/ncer_abstracts/index.cfm/fuseaction/display.abstractDetail/abstract/7384/report/0
- Kaste PJ, Rice BM (2004) Novel energetic materials for the future force: the army pursues the next generation of propellant and explosives. AMPTIAC Q 8:84–90Google Scholar
- Knox JP (1995) The extracellular-matrix in higher-plants. 4. Developmentally- regulated proteoglycans and glycoproteins of the plant-cell surface. J FASEB 9:1004–1012Google Scholar
- Meng X, Dadachov M, Korfiatis GP, Christodoulatos C (2005) Methods of preparing a surface-activated titanium oxide product and of using same in water treatment processes. U.S. Patent Application Number 6,919,029Google Scholar
- Organisation for Economic Cooperation and Development (1984) Algal growth inhibition test. OECD guidelines for testing of chemicals 201, Paris, FranceGoogle Scholar
- Tarte P (1967) Infra-red spectra of inorganic aluminates and characteristic vibrational frequencies of AlO4 tetrahedra and AlO6 octahedra. Spectrochim Acta 23A:2127–2143Google Scholar
- The Royal Society & The Royal Academy of Engineering (2004) Nanoscience and nanotechnologies: opportunities and uncertainties. Royal Society Publications, LondonGoogle Scholar
- U.S. Environmental Protection Agency Nanotechnology White Paper (2005) http://www.epa.gov/osa/pdfs/EPA_nanotechnology_white_paper_external_review_draft_12-02-2005.pdf