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Journal of Nanoparticle Research

, Volume 13, Issue 6, pp 2295–2303 | Cite as

Transportation of drug–gold nanocomposites by actinomyosin motor system

  • Harsimran KaurEmail author
  • Archana Chaudhary
  • Inderpreet Kaur
  • Kashmir Singh
  • Lalit M. Bharadwaj
Research Paper

Abstract

Nanotechnology is playing an important role in drug delivery to overcome limitations of conventional drug delivery systems in terms of solubility, in vivo stability, pharmacokinetics, and bio-distribution. The controlled transportation of drug into the cell and within the cell is a major challenge to be addressed. Cellular molecular motors have been exploited for their cargo carrying capacity for various applications including engineering and health care. Combination of nanotechnology and biomolecular motors can address some of the challenges in drug delivery. In the present study, transportation of drug nanocomposites has been demonstrated. Nanocomposites of 6-mercaptopurine and levodopa drugs (cancer and Parkinson’s disease, respectively) were prepared with gold nanoparticles (GNPs) by covalent attachment and these nanocomposites were attached to actin filaments. These nanocomposites were in-turn transported by actin filaments on myosin tracks. Characterization of drug nanocomposites formation was done by UV–Vis spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and confocal microscopy. GNP composites of 6-mercaptopurine and levodopa were formed by sulfide and amide bond formation, respectively. Average velocity of actin filament attached to nanocomposites was found to be 3.17 and 3.89 μm/s for levodopa and 6-mercaptopurine, respectively, as compared to actin filaments with velocity of 4.0–6.0 μm/s. Three concepts have been proposed for the study of drug transportation into the cell based on polycationic complex formation, interaction of actin with cellular myosin and Biomolecular Adaptor for Retrograde Transport (BART) technology. The aspects of this study heads toward the development of an approach to utilize molecular motors for nanoscale transportation endogenously.

Keywords

Actin Myosin Gold nanoparticle Levodopa 6-Mercactopurine Nanocomposites Nanobiotechnology Nanomedicine 

Notes

Acknowledgments

This work was supported by Department of Science and Technology (DST), Govt. of India. We are also thankful to Dr. Amit Sharma, Dr. Akashdeep, Suresh Kumar, and Deepak Kukkar for their valuable guidance and suggestions. One of us authors (Harsimran Kaur) thanks University Grant Commission (UGC), Govt. of India for providing Senior Research fellowship (SRF).

Supplementary material

11051_2010_9987_MOESM1_ESM.doc (24 kb)
(DOC 24 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Harsimran Kaur
    • 1
    Email author
  • Archana Chaudhary
    • 1
  • Inderpreet Kaur
    • 1
  • Kashmir Singh
    • 2
  • Lalit M. Bharadwaj
    • 1
  1. 1.Biomolecular Electronics and Nanotechnology Division (BEND), Central Scientific Instruments Organization (CSIO)Council of Scientific and Industrial Research (CSIR)ChandigarhIndia
  2. 2.Department of BiotechnologyPanjab UniversityChandigarhIndia

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