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Journal of Nanoparticle Research

, Volume 13, Issue 4, pp 1573–1580 | Cite as

Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

  • Bernard MasereelEmail author
  • Mustapha Dinguizli
  • Caroline Bouzin
  • Nicolas Moniotte
  • Olivier Feron
  • Bernard Gallez
  • Thierry Vander Borght
  • Carine Michiels
  • Stéphane Lucas
Research Paper

Abstract

Nanoscale materials are used in the biomedical field for magnetic resonance imaging, protein detection and drug/gene delivery. Gold nanoparticles (AuNPs) are particularly investigated in cancer treatment and imaging. In this study, we described a simple and reliable liquid method to coat AuNPs (diameter: 21 nm) layer-by-layer with alternative cationic polyallylamine and anionic polystyrenesulfonate. The C-terminal amino acid of the antibody directed against anti-bovine serum albumin was activated by EDC/NHS, and then condensed with the amino functions of the external polyallylamine layer. An ELISA test confirmed that the antigen recognition of the bioconjugate antibody was conserved. This AuNP coating and the covalently coupling could be used as a generic process for binding other specific antibodies, particularly those overexpressed in cancer cells and angiogenesis.

Keywords

Gold Nanoparticles Polymer Antibody Polyallylamine Polystyrenesulfonate Nanomedicine Drug delivery 

Notes

Acknowledgment

This study is supported by Walloon Region (Targan Project, grant 516071). C Michiels is a Research Associate of the Belgian FNRS.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Bernard Masereel
    • 1
    Email author
  • Mustapha Dinguizli
    • 1
  • Caroline Bouzin
    • 2
  • Nicolas Moniotte
    • 1
  • Olivier Feron
    • 2
  • Bernard Gallez
    • 2
  • Thierry Vander Borght
    • 3
  • Carine Michiels
    • 4
  • Stéphane Lucas
    • 5
  1. 1.Drug Design and Discovery CenterFUNDP, University of NamurNamurBelgium
  2. 2.Pole of Pharmacology and TherapeuticsUniversity of LouvainLouvainBelgium
  3. 3.Mont-Godinne HospitalUniversity of LouvainLouvainBelgium
  4. 4.Department of BiologyFUNDP, University of NamurNamurBelgium
  5. 5.Research Center in Physics of Matter and RadiationFUNDP, University of NamurNamurBelgium

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