Journal of Nanoparticle Research

, Volume 13, Issue 7, pp 2981–2988 | Cite as

Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity

  • Garima SinghalEmail author
  • Riju Bhavesh
  • Kunal Kasariya
  • Ashish Ranjan Sharma
  • Rajendra Pal Singh
Research paper


Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, biosynthesis of stable silver nanoparticles was done using Tulsi (Ocimum sanctum) leaf extract. These biosynthesized nanoparticles were characterized with the help of UV–vis spectrophotometer, Atomic Absorption Spectroscopy (AAS), Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Stability of bioreduced silver nanoparticles was analyzed using UV–vis absorption spectra, and their antimicrobial activity was screened against both gram-negative and gram-positive microorganisms. It was observed that O. sanctum leaf extract can reduce silver ions into silver nanoparticles within 8 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size range 4–30 nm possessing antimicrobial activity suggesting their possible application in medical industry.


Ocimum sanctum Silver nanoparticles XRD AAS DLS FTIR TEM Antimicrobial activity Nanomedicine 



We heartly acknowledge Dr. A. K. Chauhan for their support and providing facilities for the fulfillment of this study. We also acknowledge Dr. Richa Krishna for her guidance.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Garima Singhal
    • 1
    Email author
  • Riju Bhavesh
    • 1
  • Kunal Kasariya
    • 1
  • Ashish Ranjan Sharma
    • 2
  • Rajendra Pal Singh
    • 1
  1. 1.Amity Institute of Nanotechnology, Amity University Uttar PradeshNoidaIndia
  2. 2.Infectious Disease Medical Research Center, College of MedicineHallym UniversityChuncheonSouth Korea

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