Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity
Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, biosynthesis of stable silver nanoparticles was done using Tulsi (Ocimum sanctum) leaf extract. These biosynthesized nanoparticles were characterized with the help of UV–vis spectrophotometer, Atomic Absorption Spectroscopy (AAS), Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Stability of bioreduced silver nanoparticles was analyzed using UV–vis absorption spectra, and their antimicrobial activity was screened against both gram-negative and gram-positive microorganisms. It was observed that O. sanctum leaf extract can reduce silver ions into silver nanoparticles within 8 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size range 4–30 nm possessing antimicrobial activity suggesting their possible application in medical industry.
KeywordsOcimum sanctum Silver nanoparticles XRD AAS DLS FTIR TEM Antimicrobial activity Nanomedicine
We heartly acknowledge Dr. A. K. Chauhan for their support and providing facilities for the fulfillment of this study. We also acknowledge Dr. Richa Krishna for her guidance.
- Arangasamy L, Munusamy V (2008) Tapping the unexploited plant resources for the synthesis of silver nanoparticles. Afr J Biotechnol 7(17):3162–3165Google Scholar
- Crabtree JH, Brruchette RJ, Siddiqi Ra, Huen IT, Handott LL, Fishman A (2003) The efficacy of silver-ion implanted catheters in reducing peritoneal dialysis-related infections. Perit Dial Int 23(4):368–374Google Scholar
- Klug HP, Alexander LE (1967) X-ray diffraction procedures for polycrystalline and amorphous materials. Wiley, New YorkGoogle Scholar
- Mayr-Harting A, Hedges A, Berkeley R, eds (1972) Methods for studying bactericides. Academic Press, New York, p 74Google Scholar
- Mukherjee P, Ahmad A, Mandal DS, Senapati S, Sainkar R, Khan MI, Parishcha R, Ajaykumar PV, Alam M, Kumar R, Sastry M (2001) Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis. Nano Lett 1:515–519CrossRefGoogle Scholar
- Sathyavathi R, Krishna MB, Rao SV, Saritha R, Rao DN (2010) Biosynthesis of silver nanoparticles using Coriandrum Sativum leaf extract and their application in nonlinear optics. Adv Sci Lett 3:1–6Google Scholar
- Spring H, Schleifer KH (1995) Diversity of magnetotactic bacteria. Syst Appl Microbiol 18(2):147–153Google Scholar