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Journal of Nanoparticle Research

, Volume 13, Issue 7, pp 2759–2767 | Cite as

Inhibition effects of protein-conjugated amorphous zinc sulfide nanoparticles on tumor cells growth

  • Ying Cao
  • Hua-Jie Wang
  • Cui Cao
  • Yuan-Yuan Sun
  • Lin Yang
  • Bao-Qing Wang
  • Jian-Guo Zhou
Research paper

Abstract

In this article, a facile and environmentally friendly method was applied to fabricate BSA-conjugated amorphous zinc sulfide (ZnS) nanoparticles using bovine serum albumin (BSA) as the matrix. Transmission electron microscopy analysis indicated that the stable and well-dispersed nanoparticles with the diameter of 15.9 ± 2.1 nm were successfully prepared. The energy dispersive X-ray, X-ray powder diffraction, Fourier transform infrared spectrograph, high resolution transmission electron microscope, and selected area electron diffraction measurements showed that the obtained nanoparticles had the amorphous structure and the coordination occurred between zinc sulfide surfaces and BSA in the nanoparticles. In addition, the inhibition effects of BSA-conjugated amorphous zinc sulfide nanoparticles on tumor cells growth were described in detail by cell viability analysis, optical and electron microscopy methods. The results showed that BSA-conjugated amorphous zinc sulfide nanoparticles could inhibit the metabolism and proliferation of human hepatocellular carcinoma cells, and the inhibition was dose dependent. The half maximal inhibitory concentration (IC50) was 0.36 mg/mL. Overall, this study suggested that BSA-conjugated amorphous zinc sulfide nanoparticles had the application potential as cytostatic agents and BSA in the nanoparticles could provide the modifiable site for the nanoparticles to improve their bioactivity or to endow them with the target function.

Keywords

BSA Amorphous zinc sulfide Nanoparticles Aqueous chemistry method Tumor cells Nanomedicine 

Notes

Acknowledgments

This study was financially supported by the National Science Foundation of China (20971039, 20771036 and 20871042), the National Key Basic Research and Development Program of China (2009CB626610), Key Foundation Project from Education Ministry of China (207070), and the National College Students Innovation Experiment Program of China (2009039).

Conflict of interest

The authors have no conflicts of interest.

References

  1. Anand KV, Chinnu MK, Kumar RM, Mohan R, Jayavel R (2010) Thermal stability and optical properties of HMTA capped zinc sulfide nanoparticles. J Alloys Compd 496:665–668CrossRefGoogle Scholar
  2. Battaglia A, Ghidini S, Campanini G, Spaggiari R (2005) Heavy metal contamination in little owl (Athene noctua) and common buzzard (Buteo buteo) from northern Italy. Ecotoxicol Environ Saf 60:61–66CrossRefGoogle Scholar
  3. Brannon-Peppas L, Blanchette JO (2004) Nanoparticle and targeted systems for cancer therapy. Adv Drug Deliv Rev 56:1649–1659CrossRefGoogle Scholar
  4. Cheng Y, Samia AC, Malcolm JL, Kenney E, Resnick A, Burda C (2010) Delivery and efficacy of a cancer drug as a function of the bond to the gold nanoparticle surface. Langmuir 26(4):2248–2255CrossRefGoogle Scholar
  5. Goswami N, Sen P (2004) Water-induced stabilization of ZnS nanoparticles. Solid State Commun 132:791–794CrossRefGoogle Scholar
  6. Guo YM, Zhang J, Yang L, Wang HJ, Wang FF, Zheng Z (2010) Syntheses of amorphous and crystalline cupric sulfide nanoparticles and study on the specific activities on different cells. Chem Commun 46:3493–3495CrossRefGoogle Scholar
  7. Jamieson T, Bakhshi R, Petrova D, Pocock R, Imani M, Seifalian AM (2007) Biological applications of quantum dots. Biomaterials 28:4717–4732CrossRefGoogle Scholar
  8. Jiang CL, Zhang WQ, Zou GF, Yu WC, Qian YT (2007) Hydrothermal synthesis and characterization of ZnS microspheres and hollow nanospheres. Mater Chem Phys 103:24–27CrossRefGoogle Scholar
  9. Khiew PS, Radiman S, Huang NM, Ahmad MS, Nadarajah K (2005) Preparation and characterization of ZnS nanoparticles synthesized from chitosan laurate micellar solution. Mater Lett 59:989–993CrossRefGoogle Scholar
  10. Kusakabe T, Nakajima K, Nakazato K, Suzuki K, Takada H, Satoh T, Oikawa M, Arakawa K, Nagamine T (2008) Changes of heavy metal, metallothionein and heat shock proteins in Sertoli cells induced by cadmium exposure. Toxicol In Vitro 22:1469–1475CrossRefGoogle Scholar
  11. Lee SS, Byun KT, Park JP, Kim SK, Lee JC, Chang SK, Kwak HY, Shim IW (2008) Homogeneous ZnS coating onto TiO2 nanoparticles by a simple one pot sonochemical method. Chem Eng J 139:194–197CrossRefGoogle Scholar
  12. Mathew ME, Mohan JC, Manzoor K, Nair SV, Tamura H, Jayakumar R (2010) Folate conjugated carboxymethyl chitosan-manganese doped zinc sulphide nanoparticles for targeted drug delivery and imaging of cancer cells. Carbohydr Polym 80:442–448CrossRefGoogle Scholar
  13. Mehdizadeh M, Kermanian F, Farjah G (2008) Schwann cell injuries of radial nerve after lead (Pb) exposure in rats. Pathophysiology 15:13–17CrossRefGoogle Scholar
  14. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63CrossRefGoogle Scholar
  15. Nam J, Won N, Jin H, Chung H, Kim S (2009) pH-induced aggregation of gold nanoparticles for photothermal cancer therapy. J Am Chem Soc 131(38):13639–13645CrossRefGoogle Scholar
  16. Ni Shuilleabhain SN, Mothersill C, Sheehan D, O’Brien NM, O’Halloran J, Van Pelt FNAM, Davoren M (2004) In vitro cytotoxicity testing of three zinc metal salts using established fish cell lines. Toxicol In Vitro 18:365–376CrossRefGoogle Scholar
  17. Perez-Lopez M, Cid F, Oropesa AL, Hidalgo LE, Lopez-Beceiro A, Soler F (2006) Heavy metal and arsenic content in seabirds affected by the Prestige oil spill on the Galician coast (NW Spain). Sci Total Environ 359(1–3):209–220Google Scholar
  18. Rema Devi BS, Raveendran R, Vaidyan AV (2007) Synthesis and characterization of Mn2+-doped ZnS nanoparticles. Pramana. J Phys 68(4):679–687Google Scholar
  19. Sanpera C, Morera M, Ruiz X, Jover L (2000) Variability of mercury and selenium levels in clutches of Audouin’s gulls (Larus audouinii) breeding at the Chafarinas Islands, Southwest Mediterranean. Arch Environ Contam Toxicol 39:119–123Google Scholar
  20. Sayes CM, Wahi R, Kurian PA, Liu Y, West JL, Ausman KD, Warheit DB, Colvin VL (2006) Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. Toxicol Sci 92:174–185CrossRefGoogle Scholar
  21. Schroeder JE, Shweky I, Shmeeda H, Banin U, Gabizon A (2007) Folate-mediated tumor cell uptake of quantum dots entrapped in lipid nanoparticles. J Control Release 124(1–2):28–34CrossRefGoogle Scholar
  22. Seoudi R, Shabaka A, Eisa WH, Anies B, Farage NM (2010) Effect of the prepared temperature on the size of CdS and ZnS nanoparticles. Physica B 405:919–924CrossRefGoogle Scholar
  23. Skrabalak SE, Chen J, Au L, Lu X, Li X, Xia Y (2007) Gold nanocages for biomedical applications. Adv Mater 19:3177–3184CrossRefGoogle Scholar
  24. Steinebach OM, Wolterbeek HT (1993) Effects of zinc on rat hepatoma HTC cells and primary cultured rat hepatocytes. Toxicol Appl Pharmacol 118:245–254CrossRefGoogle Scholar
  25. Valee BL, Auld DA (1990) Zinc coordination, function, and structure of zinc enzymes and other proteins. Biochemistry 29(24):5647–5659CrossRefGoogle Scholar
  26. Walther UI (2004) Changes in the glutathione system of lung cell lines after treatment with hydrocortisone. Arch Toxicol 78:402–409CrossRefGoogle Scholar
  27. Walther UI, Walther SC, Temruck O (2007) Effect of enlarged glutathione on zinc-mediated toxicity in lung-derived cell lines. Toxicol In Vitro 21:380–386CrossRefGoogle Scholar
  28. Wang B, Feng WY, Wang TC, Jia G, Wang M, Shi JW, Zhang F, Zhao YL, Chai ZF (2006) Acute toxicity of nano- and micro-scale zinc powder in healthy adult mice. Toxicol Lett 161:115–123CrossRefGoogle Scholar
  29. Wang HJ, Yang L, Yang HY, Wang K, Yao WG, Jiang K, Huang XL, Zheng Z (2010) Antineoplastic activities of protein-conjugated silver sulfide nano-crystals with different shapes. J Inorg Biochem 104:87–91CrossRefGoogle Scholar
  30. Warheit DB, Webb TR, Sayes CM, Colvin VL, Reed KL (2006) Pulmonary instillation studies with nanoscale TiO2 rods and dots in rats: toxicity is not dependent upon particle size and surface area. Toxicol Sci 91:227–236CrossRefGoogle Scholar
  31. Warheit DB, Webb TR, Colvin VL, Reed KL, Sayes CM (2007) Pulmonary bioassay studies with nanoscale and fine-quartz particles in rats: toxicity is not dependent upon particle size but on surface characteristics. Toxicol Sci 95:270–280CrossRefGoogle Scholar
  32. Wihelm B, Walther UI, Fichtl B (2001) Effects of zinc chloride on glutathione and glutathione synthesis rates in various lung cell lines. Arch Toxicol 75:388–394CrossRefGoogle Scholar
  33. Yang L, Shen Q, Zhou J, Jiang K (2006) Biomimetic synthesis of CdS nanocrystals in aqueous solution of pepsin. Mater Chem Phys 98:125–130CrossRefGoogle Scholar
  34. Yang L, Wang HJ, Yang HY, Liu SH, Zhang BF, Wang K, Ma XM, Zheng Z (2008) Shape-controlled synthesis of protein-conjugated silver sulfide nanocrystals and study on the inhibition of tumor cell viability. Chem Commun 2995–2997Google Scholar
  35. Zhang J, Ma X, Guo Y, Yang L, Shen Q, Wang H, Ma Z (2010) Size-controllable preparation of bovine serum albumin-conjugated. Mater Chem Phys 119:112–117CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ying Cao
    • 1
  • Hua-Jie Wang
    • 1
  • Cui Cao
    • 1
  • Yuan-Yuan Sun
    • 1
  • Lin Yang
    • 1
  • Bao-Qing Wang
    • 1
  • Jian-Guo Zhou
    • 1
  1. 1.College of Chemistry and Environmental ScienceHenan Normal UniversityXinxiangPeople’s Republic of China

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