Journal of Nanoparticle Research

, Volume 13, Issue 1, pp 263–271 | Cite as

Single enzyme nanoparticle for biomimetic CO2 sequestration

  • Renu Yadav
  • Nitin Labhsetwar
  • Swati Kotwal
  • Sadhana Rayalu
Research Paper
First Online:
Received:
Accepted:

Abstract

Nanoparticle technology is being increasingly used in environmental sciences. We prepared single enzyme nanoparticle (SEN) by modifying the surface of carbonic anhydrase (CA) with a thin layer of organic/inorganic hybrid polymer. SEN-CA appears to be improving the stability of free enzyme. CA, as ubiquitously found enzyme, is involved in gaseous CO2 sequestration and is being looked as a promising candidate for combating global warming. We report here physical characterization of SEN-CA using transmission electron microscope (TEM), Fourier-transform infrared analysis (FTIR), X-ray diffraction analysis (XRD), and energy dispersive X-ray (EDX). Average size of SEN-CA particles appears to be in the range of 70–80 nm. We also report the effect of SEN formation on the kinetic parameters of free CA such as Michaelis–Menten constant (K m), maximum reaction velocity (V max), and storage stability of free CA and SEN-CA. The V max of SEN-CA (0.02857 mmol/min/mg) and free enzyme (0.02029 mmol/min/mg) is almost similar. K m has decreased from 6.143 mM for SEN-CA to 1.252 mM for free CA. The stabilization of CA by SEN formation results in improved the half-life period (up to 100 days). The formation of carbonate was substantiated by using gas chromatography (GC). The conversion of CO2 to carbonate was 61 mg of CaCO3/mg of CA and 20.8 mg of CaCO3/mg of CA using SEN-CA and free CA, respectively.

Keywords

Single enzyme nanoparticle Carbonic anhydrase Carbonation reaction Half-life period Environmental remediation 
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Notes

Acknowledgments

This study was carried out under the Supra Institutional Project [SIP-16 (4.2)], Council of Scientific and Industrial Research (CSIR) and the Department of Biotechnology (DBT), New Delhi, sponsored project. We are grateful to The Director, National Environmental Engineering Research Institute (NEERI), Nagpur for providing constant encouragement and help. We are thankful to Dr. T. Satyanarayanan, University Department of Microbiology, New Delhi, for providing partially purified carbonic anhydrase and Dr. Peshwe, Visvesvaraya National Institute of Technology (VNIT), Nagpur for characterization of materials. I acknowledge the help of Prof. Hata, Kyushu University, Chikushi Campus, Fukuoka, Japan in providing TEM image of SEN-CA.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Renu Yadav
    • 1
  • Nitin Labhsetwar
    • 1
  • Swati Kotwal
    • 2
  • Sadhana Rayalu
    • 1
  1. 1.Environmental Materials UnitNational Environmental Engineering Research Institute (NEERI), CSIRNagpurIndia
  2. 2.Department of BiochemistryR.T.M. Nagpur UniversityNagpurIndia

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