Highly bacterial resistant silver nanoparticles: synthesis and antibacterial activities
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In this article, we describe a simple one-pot rapid synthesis route to produce uniform silver nanoparticles by thermal reduction of AgNO3 using oleylamine as reducing and capping agent. To enhance the dispersal ability of as-synthesized hydrophobic silver nanoparticles in water, while maintaining their unique properties, a facile phase transfer mechanism has been developed using biocompatible block co-polymer pluronic F-127. Formation of silver nanoparticles is confirmed by X-ray diffraction (XRD), transmission electron microscopy (TEM) and UV–vis spectroscopy. Hydrodynamic size and its distribution are obtained from dynamic light scattering (DLS). Hydrodynamic size and size distribution of as-synthesized and phase transferred silver nanoparticles are 8.2 ± 1.5 nm (σ = 18.3%) and 31.1 ± 4.5 nm (σ = 14.5%), respectively. Antimicrobial activities of hydrophilic silver nanoparticles is tested against two Gram positive (Bacillus megaterium and Staphylococcus aureus), and three Gram negative (Escherichia coli, Proteus vulgaris and Shigella sonnei) bacteria. Minimum inhibitory concentration (MIC) values obtained in the present study for the tested microorganisms are found much better than those reported for commercially available antibacterial agents.
KeywordsSilver nanoparticles Pluronic F-127 Antibacterial activity Minimum inhibitory concentration Nanomedicine
The work was carried out under Ramanna Fellowship Project (RVM) and FTPYS Project (AKV) awarded by the Department of Science and Technology (DST), New Delhi. Authors are thankful to Microbiology Department of Sir P.P. Institute of Science, Bhavnagar University, India for providing microorganisms and Professor S.K. Menon, Department of Chemistry, Gujarat University, India for her help in DLS measurement.
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