Immunological impact of magnetic nanoparticles (Ferucarbotran) on murine peritoneal macrophages
Ferucarbotran, a clinically used superparamagnetic iron oxide, is widely developed as a magnetic resonance imaging (MRI) contrast agent and has the potential to improve the monitoring of macrophage recirculation in vivo. However, the biological effect of Ferucarbotran or magnetic nanoparticles (MNPs) on macrophage is not clearly understood yet. This study is aimed to examine the immunological impact of Ferucarbotran toward murine peritoneal macrophages. Cells treated with Ferucarbotran demonstrated a dose–responsive increase of granularity in the cytoplasm. After 24 h of incubation, viability and cytotoxicity in macrophages treated with 200 μg Fe/mL of Ferucarbotran were not affected. Macrophages loaded with Ferucarbotran above 100 μg Fe/mL showed a significant (p < 0.01) increase in cytokine (TNF-α, IL-1β, IL-6) secretion and mRNA expression, followed by nitric oxide (NO) secretion and iNOS mRNA expression. Chemotactic responses of Ferucarbotran-preloaded macrophages toward CX3CL1 were significantly (p < 0.05) lower than those of untreated macrophages. Taking together, Ferucarbotran at high dose (100 μg Fe/mL) could induce murine peritoneal macrophages activation in pro-inflammatory cytokine secretion and NO production.
KeywordsSuperparamagnetic iron oxide Magnetic resonance imaging Cytotoxicity Inflammation Cell activation Chemotaxis Nanomedicine
Magnetic resonance imaging
Superparamagnetic iron oxide
Ultrasmall superparamagnetic iron oxide
This study was supported by grants from the National Science Council, Taiwan (NSC-96-2815-C-002-080-B).
- Muller K, Skepper JN, Tang TY et al (2008) Atorvastatin and uptake of ultrasmall superparamagnetic iron oxide nanoparticles (Ferumoxtran-10) in human monocyte-macrophages: implications for magnetic resonance imaging. Biomaterials 29:2656–2662. doi: 10.1016/j.biomaterials.2008.03.006 CrossRefPubMedGoogle Scholar