Fusarium solani: a novel biological agent for the extracellular synthesis of silver nanoparticles
- 1.8k Downloads
We report extracellular biosynthesis of silver nanoparticles by Fusarium solani (USM-3799), a phytopathogen causing disease in onion, when challenged with 1 mM silver nitrate (AgNO3). The formation of nanoparticles was characterized by visual observation followed by UV–Vis spectrophotometric analysis, which showed a peak at about 420 nm, which is very specific for silver nanoparticles. Further analysis carried out by Fourier Transform Infrared Spectroscopy (FTIR), provides evidence for the presence of proteins as capping agent, which helps in increasing the stability of the synthesized silver nanoparticles. Transmission Electron Microscopy (TEM) investigations confirmed that silver nanoparticles were formed. The synthesized silver nanoparticles were found to be polydispersed, spherical in the range of 5–35 nm with average diameter of 16.23 nm. Extracellular synthesis of nanoparticles could be highly advantageous from the point of view of synthesis in large quantities and easy downstream processing.
KeywordsExtracellular Fusarium solani TEM Eco-friendly Downstream processing Antimicrobial agent Nanobiotechnology
We thank Prof. Carsten Sönnichsen and Dr. Sebastien Pierrat, Johannes Gutenberg University of Mainz, Germany for their help in TEM analysis and we are also thankful to Prof. Baharuddin Salleh, University Sains Malaysia, Malaysia for confirming the identification of F. solani.
- Cao G (ed) (2004) Nanostructures and nanomaterials: synthesis, properties, and applications. Imperial College Press, LondonGoogle Scholar
- Frankel RB, Blakemore RP (1991) Iron biominerals. Plenum, New YorkGoogle Scholar
- Hayat MA (1989) Colloidal gold: principles, methods and applications. Academic Press, CaliforniaGoogle Scholar
- Pandey G, Srivastava SK (2006) A novel synthetic method for the preparation of CuS, and CdS nanochains. Synthesis and reactivity in inorganic, metal organic and nano metal. Chemistry (Easton) 36:663–666Google Scholar
- Sastry M, Ahmad A, Khan MI, Kumar R (2003) Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr Sci 85:162–170Google Scholar
- Seifert K (1996) Fusarium interactive key. Agriculture and Agri Food Canada, pp 1–65Google Scholar
- Spring H, Schleifer KH (1995) Diversity of magnetotactic bacteria. Syst Appl Microbiol 18:147–153Google Scholar
- Sushil KS, Mamta P (2003) Microbially synthesized bioactive nanoparticles and their formulation active against human pathogenic fungi. Rev Adv Mater Sci 5:501–509Google Scholar