Advertisement

Journal of Nanoparticle Research

, Volume 10, Issue 3, pp 393–400 | Cite as

The detection of HBV DNA with gold-coated iron oxide nanoparticle gene probes

  • Dong Xi
  • XiaoPing LuoEmail author
  • QiangHua Lu
  • KaiLun Yao
  • ZuLi Liu
  • Qin NingEmail author
Research Paper

Abstract

Gold-coated iron oxide nanoparticle Hepatitis B virus (HBV) DNA probes were prepared, and their application for HBV DNA measurement was studied. Gold-coated iron oxide nanoparticles were prepared by the citrate reduction of tetra-chloroauric acid in the presence of iron oxide nanoparticles which were added as seeds. With a fluorescence-based method, the maximal surface coverage of hexaethiol 30-mer oligonucleotides and the maximal percentage of hybridization strands on gold-coated iron oxide nanoparticles were (120 ± 8) oligonucleotides per nanoparticle, and (14 ± 2%), respectively, which were comparable with those of (132 ± 10) and (22 ± 3%) in Au nanoparticle groups. Large network aggregates were formed when gold-coated iron oxide nanoparticle HBV DNA gene probe was applied to detect HBV DNA molecules as evidenced by transmission electron microscopy and the high specificity was verified by blot hybridization. Our results further suggested that detecting DNA with iron oxide nanoparticles and magnetic separator was feasible and might be an alternative effective method.

Keywords

Hepatitis B virus DNA DNA probe Nanoparticle Hybridization Coatings Nanomedicine 

Notes

Acknowledgement

This work was supported by the National Science Foundation of China (NSFC) operating fund 30571643 and 30672380, National Key Basic Research Program of China (2005CB522901, 2005CB522507), Clinical Key Program of Ministry of Health of China and National High Technology Program (2002AA302202, 2003DF000034, 2003CB514112, 20041003068-05).

References

  1. Bao YP, Huber M, Wei TF, Marla SS, Storhoff JJ, Muller UR (2005) SNP identification in unamplified human genomic DNA with gold nanoparticle probes. Nucleic Acids Res 33:e15CrossRefGoogle Scholar
  2. Bendayan M (2001) Worth its weight in gold. Science 291:1363–1365CrossRefGoogle Scholar
  3. Cui YL, Wang YN, Hui WL, Zhang ZF, Xin XF, Chen C (2005) The synthesis of goldmag nano-particles and their application for antibody immobilization. Biomed Microdevices 7(2):153–156CrossRefGoogle Scholar
  4. Daniel MC, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104:293–346CrossRefGoogle Scholar
  5. Demers LM, Mirkin CA, Mucic RC, Peynolds RA, Letsinger RL, Elghanian R, Viswanadham G (2000) A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-cappped oligonucleotides bound to gold thin films and nanoparticles. Anal Chem 72:5535–5541CrossRefGoogle Scholar
  6. Elghanian R, Storhoff JJ, Mucic RC, Letsinger RL, Mirkin CA (1997) Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. Science 277:1078–1081CrossRefGoogle Scholar
  7. Hacia JG, Brody LC, Chee MS, Foder SP, Collins FS (1996) Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two-colour fluorescence analysis. Nature Genet 14:441–447CrossRefGoogle Scholar
  8. Huber M, Wei TF, Muller UR, Lefebvre PA, Marla SS, Bao YP (2004) Gold nanoparticle probe-based gene expression analysis with unamplified total human RNA. Nucleic Acids Res 32:e137CrossRefGoogle Scholar
  9. Jennifer LL, Fleming DA, Stone MB, Schiffer P, Williams ME (2004) Synthesis of Fe oxide core/Au shell nanoparticles by iterative hydroxylamine seeding. Nano Lett 4:719–723CrossRefGoogle Scholar
  10. Lu QH, Yao KL, Xi D, Liu ZL, Luo XP, Ning Q (2006) Synthesis and characterization of composite nanoparticles comprised of gold shell and magnetic core/cores. J Magnetism Magn Mater 301:44–46CrossRefGoogle Scholar
  11. Lucarelli F, Marrazza G, Turner APF, Mascini M (2004) Carbon and gold electrodes as electrochemical transducers for DNA hybridization sensors. Biosens Bioelectron 19:515–530CrossRefGoogle Scholar
  12. Mansfield ES, Worley JM, McKenzie SE, Surrey S, Rappaport E, Fortina P (1995) Nucleic acid detection using non-radioactive labelling methods. Mol Cell Probes 9(3):145–156CrossRefGoogle Scholar
  13. Micales BK, Lyons GE (2001) In situ hybridization: use of 35S-labeled probes on paraffin tissue sections. Methods 23(4):313–323CrossRefGoogle Scholar
  14. Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ (1996) A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382:607–609CrossRefGoogle Scholar
  15. Pena SRN, Raina S, Goodrich GP, Fedoroff NV, Keating CD (2002) Hybridization and enzymatic extension of au nanoparticle-bound oligonucleotides. J Am Chem Soc 124:7314–7323CrossRefGoogle Scholar
  16. Penn SG, He L, Natan MJ (2003) Nanoparticles for bioanalysis. Curr Opin Chem Biol 7:609–615CrossRefGoogle Scholar
  17. Wang YF, Pang DW, Zhang ZL, Zheng HZ, Cao JP, Shen JT (2003) Visual gene diagnosis of HBV and HCV based on nanoparticle probe amplification and silver staining enhancement. J Med Virol 70:205–211CrossRefGoogle Scholar
  18. Weng J, Ren J (2006) Luminescent quantum dots: a very attractive and promising tool in biomedicine. Curr Med Chem 13(8):897–909CrossRefGoogle Scholar
  19. Yuan J, Wang G (2005) Lanthanide complex-based fluorescence label for time-resolved fluorescence bioassay. J Fluoresc 15(4):559–568CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Laboratory of Infectious Immunology, Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R. China
  2. 2.Department of Pediatrics, Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R. China
  3. 3.Department of PhysicsHuazhong University of Science and TechnologyWuhanP.R. China
  4. 4.Department of Infectious Disease, Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R. China

Personalised recommendations