Journal of Nanoparticle Research

, Volume 10, Issue 3, pp 393–400

The detection of HBV DNA with gold-coated iron oxide nanoparticle gene probes

Research Paper

DOI: 10.1007/s11051-007-9263-1

Cite this article as:
Xi, D., Luo, X., Lu, Q. et al. J Nanopart Res (2008) 10: 393. doi:10.1007/s11051-007-9263-1

Abstract

Gold-coated iron oxide nanoparticle Hepatitis B virus (HBV) DNA probes were prepared, and their application for HBV DNA measurement was studied. Gold-coated iron oxide nanoparticles were prepared by the citrate reduction of tetra-chloroauric acid in the presence of iron oxide nanoparticles which were added as seeds. With a fluorescence-based method, the maximal surface coverage of hexaethiol 30-mer oligonucleotides and the maximal percentage of hybridization strands on gold-coated iron oxide nanoparticles were (120 ± 8) oligonucleotides per nanoparticle, and (14 ± 2%), respectively, which were comparable with those of (132 ± 10) and (22 ± 3%) in Au nanoparticle groups. Large network aggregates were formed when gold-coated iron oxide nanoparticle HBV DNA gene probe was applied to detect HBV DNA molecules as evidenced by transmission electron microscopy and the high specificity was verified by blot hybridization. Our results further suggested that detecting DNA with iron oxide nanoparticles and magnetic separator was feasible and might be an alternative effective method.

Keywords

Hepatitis B virus DNA DNA probe Nanoparticle Hybridization Coatings Nanomedicine 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Laboratory of Infectious Immunology, Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R. China
  2. 2.Department of Pediatrics, Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R. China
  3. 3.Department of PhysicsHuazhong University of Science and TechnologyWuhanP.R. China
  4. 4.Department of Infectious Disease, Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R. China

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